Analysis of human sera that are polyreactive in an addressable laser bead immunoassay

Clinical Immunology : the Official Journal of the Clinical Immunology Society
Marvin J FritzlerMark L Fritzler

Abstract

Following the introduction of addressable laser bead immunoassays (ALBIA) into our clinical laboratory, it was noted that certain sera would exhibit reactivity to numerous antigens in the array. To further understand the nature of this reactivity, we analyzed the reactivity of sequential sera that were identified over a 1 year period. Sera that demonstrated reactivity to 6 or more of the 8 antigens in an ALBIA kit (QuantaPlex 8: chromatin, Sm, RNP, Scl-70, ribosomal P protein, SS-A/Ro, SS-B/La, Jo-1) were tested for autoantibodies by indirect immunofluorescence (IIF) on HEp-2 cell substrates, for IgG, IgM and IgA rheumatoid factor, chromatin and ribosomal P protein by ELISA and by LINE immunoassay (LIA) and immunoblotting (IB). In one calendar year, 40/4096 (0.8%) sera analyzed in a routine clinical laboratory setting demonstrated reactivity to 6 or more antigens in the QuantaPlex 8 kits. There was no common IIF pattern that could be attributed to the polyreactive sera. There was no apparent correlation of polyreactivity with IIF titers, indeed, 4/40 (10%) sera had a negative ANA at the screening dilution of 1/160. When subjected to IB, LIA and ELISA, polyreactivity to three or more antigens was confirmed for 12/40 (30%) of ser...Continue Reading

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Citations

Feb 4, 2014·Arthritis Research & Therapy·Laurent DrouotUNKNOWN French Myositis Network [CN]
Jan 1, 2009·Expert Opinion on Medical Diagnostics·Marvin J Fritzler, Mark L Fritzler
Jul 6, 2010·Autoimmunity Reviews·Michael MahlerMarvin J Fritzler
May 22, 2007·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Eduardo BonillaAndras Perl
Oct 25, 2012·Clinical Chemistry and Laboratory Medicine : CCLM·Renato TozzoliNicola Bizzaro
Sep 18, 2007·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Rahima A BhanjiMarvin J Fritzler
Jan 1, 2014·Clinical & Developmental Immunology·M InfantinoM Mahler
Mar 14, 2008·Clinical and Vaccine Immunology : CVI·Mario Berth, Eugene Bosmans
Jul 1, 2016·The Journal of Applied Laboratory Medicine·Xiaoli DengMelissa R Snyder

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