Analysis of human thymocyte subpopulations using discontinuous gradients of albumin: precursor lymphocytes in human thymus

European Journal of Immunology
J GatienE Merler

Abstract

Precursor lymphocytes, obtained from human tissue on bovine serum albumin (BSA) gradients followed by removal of sheep erythrocyte (E)-reactive thymocytes on Ficoll, are the predominant lymphoid subpopulation found in young fetal thymuses. This subpopulation shows a progressive decline in relative numbers during fetal life, accompanied by a rise in both the relative and absolute numbers of E-reactive thymocytes. Precursor thymocytes differentiate in vitro to E-reactive cells resembling T cells in their morphology, sedimentation on BSA gradients, and response to phytohemagglutinin. Agents tending to increase intracellular concentrations of cyclic-AMP accelerate the rate of differentiation of precursor cells to lymphocytes which closely resemble T cells.

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Related Concepts

Cell Adhesion
Cell Differentiation Process
Cell Separation
Centrifugation, Density Gradient
Bucladesine, Barium (1: 1) Salt
Gestational Age
Lymphoid Cells
Phytomitogens
Receptors, Antigen, B-Cell
T-Lymphocyte

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