Abstract
Two MoAbs, independently raised against ovarian carcinoma cells and referred to as OV-TL3 and OV-TL16, display an identical reaction pattern with a membrane-associated protein in both normal and malignant ovarian cells. Also, a similar binding affinity constant and a similar number of binding sites per cell indicate that both MoAbs bind to the same antigen. Competition assays reveal that OV-TL16 is able to compete with OV-TL3 for binding to OVCAR-3 cells. Epitope mapping using a filamentous phage hexapeptide epitope library showed that both MoAbs are able to select identical phages, suggesting that their epitopes are identical or at least overlapping. However, purified polyclonal and monoclonal anti-idiotypic antibodies directed against OV-TL3 failed to recognize the OV-TL16 idiotype, indicating that the structure of the antigen-binding regions of both antibodies is distinct. This was corroborated by molecular cloning and sequencing of the variable heavy (VH) and light (VL) chain immunoglobulin regions of both MoAbs. The VH regions of both antibodies were found to be distinct, whereas the VL regions are almost identical. Computer modelling of the idiotypes suggests that the complementarity determining regions (CDR), with the ex...Continue Reading
References
Jun 5, 1992·Journal of Molecular Biology·C W Stephen, D P Lane
Oct 5, 1992·Journal of Molecular Biology·R B ChristianB A Malcolm
Feb 21, 1992·Science·J M RiniI A Wilson
Jul 27, 1990·Science·J K Scott, G P Smith
Sep 1, 1991·The Journal of Experimental Medicine·S E Stark, A J Caton
Jul 1, 1991·Nuclear Medicine Communications·L F MassugerF H Corstens
Feb 1, 1990·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·O C BoermanL G Poels
Jul 27, 1990·Science·J J DevlinP E Devlin
Mar 15, 1987·International Journal of Cancer. Journal International Du Cancer·S MiottiM I Colnaghi
Dec 20, 1988·Gene·S F Parmley, G P Smith
Oct 6, 1988·Nature·R E BruccoleriJ Novotný
Aug 15, 1970·Nature·U K Laemmli
Mar 1, 1984·Analytical Biochemistry·I HemmiläT Lövgren
Jan 7, 1994·Journal of Molecular Biology·V Böttger, E B Lane