Analysis of individual data from bead-based assays ("bead arrays")

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
James W JacobsonJohn Kettman

Abstract

Typically, bead-based assays ("bead arrays") use the mean or median value of a population of measurements to judge ligand binding or other activity, which results in a change in fluorescence intensity. Individual bead measurements are used here to calculate population parameters integral to the measurement of a bead array. Using a commercially-available instrument designed for bead array measurements, a set of beads were labeled with biotin and then titrated with PE-Streptavidin. Data were collected under normal machine conditions as well as variations. The "sensitivity" of the measurements was determined using parametric and nonparametric statistical methods as well as regression analysis over a limited range of the titration (concentration vs. response profile). Results at low ligand concentrations suggest that precise measurements with bead array systems require a large number of individual bead measurements to be acquired. Individual bead measurements should be used to determine the mean and confidence intervals for the calculated measurements. These results also apply to regression analysis of concentration-response profiles. Furthermore, features of the instrument can be manipulated to achieve increased sensitivity and de...Continue Reading

References

Jul 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I T Young
Oct 17, 1998·Cytometry·J R KettmanR J Fulton
Oct 17, 1998·Cytometry·J C Wood, R A Hoffman
Oct 17, 1998·Cytometry·H M ShapiroP G Stein
Jul 2, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Matthew M FerrisRichard A Keller

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Citations

Sep 10, 2010·Analytical and Bioanalytical Chemistry·Jia Duo, Julie A Stenken
Feb 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Joong-Ho WonRichard A Olshen
Aug 25, 2007·Theoretical Biology & Medical Modelling·Brian P HanleyR Holland Cheng
Aug 31, 2007·Theoretical Biology & Medical Modelling·Brian Hanley
Jan 30, 2008·Theoretical Biology & Medical Modelling·Brian P Hanley
Nov 8, 2008·Journal of Occupational Medicine and Toxicology·Nasr Ya Hemdan
May 14, 2009·Analytical Biochemistry·S A M MartinsG A Monteiro
Feb 14, 2014·Immunologic Research·Yael Rosenberg-HassonHolden T Maecker
Mar 15, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·S R CorrieM Trau
Mar 21, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·János SzöllosiAttila Tárnok
Nov 15, 2008·BioTechniques·Shannon BruseLinda Brzustowicz
Sep 5, 2008·Current Protocols in Cytometry·Mara R DiazJames W Jacobson
Nov 19, 2015·Journal of the International Society of Sports Nutrition·Kyle LeversRichard B Kreider

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