Analysis of infectious virus clones from two HIV-1 superinfection cases suggests that the primary strains have lower fitness.

Retrovirology
Antoinette C van der KuylMarion Cornelissen

Abstract

Two HIV-1 positive patients, L and P, participating in the Amsterdam Cohort studies acquired an HIV-1 superinfection within half a year from their primary HIV-1 infection (Jurriaans et al., JAIDS 2008, 47:69-73). The aim of this study was to compare the replicative fitness of the primary and superinfecting HIV-1 strains of both patients. The use of isolate-specific primer sets indicated that the primary and secondary strains co-exist in plasma at all time points after the moment of superinfection. Biological HIV-1 clones were derived from peripheral blood CD4 + T cells at different time point, and identified as the primary or secondary virus through sequence analysis. Replication competition assays were performed with selected virus pairs in PHA/IL-2 activated peripheral blood mononuclear cells (PBMC's) and analyzed with the Heteroduplex Tracking Assay (HTA) and isolate-specific PCR amplification. In both cases, we found a replicative advantage of the secondary HIV-1 strain over the primary virus. Full-length HIV-1 genomes were sequenced to find possible explanations for the difference in replication capacity. Mutations that could negatively affect viral replication were identified in the primary infecting strains. In patient L...Continue Reading

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Citations

Dec 14, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Marion CornelissenAntoinette C van der Kuyl
Jun 8, 2012·The Journal of Infectious Diseases·Andrew D ReddThomas C Quinn
Oct 14, 2011·Sexually Transmitted Diseases·Thana KhawcharoenpornArunee Thitithanyanont
Dec 14, 2011·Current Opinion in Infectious Diseases·Laura Waters, Erasmus Smit
Jun 24, 2011·Journal of Clinical Microbiology·Andrew D ReddUNKNOWN Rakai Health Sciences Program
Mar 7, 2012·Future Virology·Denis R ChoperaZabrina L Brumme
Jun 1, 2012·The Journal of General Virology·Nicolas LegrandMireille Centlivre
Sep 19, 2012·Current Opinion in Virology·Andrew R Wargo, Gael Kurath

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Datasets Mentioned

BETA
K03455

Methods Mentioned

BETA
PCR
nucleic acid folding
glycosylation
PMA
enzyme-linked immunosorbent assay
PCRs
transfection

Software Mentioned

CLUSTAL W
[UNK] ( Gene Network Central )
TINA
MEGA4
PerkinElmer OptiQuant
Alibaba
Geno2pheno
BioEdit Sequence Alignment Editor
TFSEARCH
mfold

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