Analysis of influenza viruses from patients clinically suspected of infection with an oseltamivir resistant virus during the 2009 pandemic in the United States

Antiviral Research
Ha T NguyenLarisa Gubareva

Abstract

During the 2009 influenza pandemic, the Centers for Disease Control and Prevention provided antiviral susceptibility testing for patients infected with suspected drug-resistant viruses. Specimens from 72 patients admitted to an intensive care unit or with a severe immunocompromising condition, who failed to clinically improve after oseltamivir treatment, were accepted for testing. Respiratory specimens were tested for the presence of the oseltamivir resistance-conferring H275Y substitution in the neuraminidase (NA) by pyrosequencing. Virus isolates propagated in MDCK cells were tested in phenotypic NA inhibition (NI) assays using licensed NA inhibitors (NAIs), zanamivir and oseltamivir, and investigational NAIs, peramivir and laninamivir. Conventional sequencing and plaque purification were conducted on a subset of viruses. Pyrosequencing data were obtained for 87 specimens collected from 58 of the 72 (81%) patients. Of all patients, 27 (38%) had at least one specimen in which H275Y was detected. Analysis of sequential samples from nine patients revealed intra-treatment emergence of H275Y variant and a shift from wildtype-to-H275Y in quasispecies during oseltamivir therapy. A shift in the H275Y proportion was observed as a resu...Continue Reading

References

Dec 17, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Frederick Hayden
Mar 4, 2009·JAMA : the Journal of the American Medical Association·Nila J DharanUNKNOWN Oseltamivir-Resistance Working Group
Dec 24, 2009·Antimicrobial Agents and Chemotherapy·Varough M DeydeLarisa V Gubareva
May 7, 2010·The New England Journal of Medicine·Edgar BautistaKarl G Nicholson
Sep 23, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ha T NguyenLarisa V Gubareva
Dec 15, 2010·Antiviral Therapy·Larisa V GubarevaAlexander I Klimov
Feb 5, 2011·Emerging Infectious Diseases·Samuel B GraitcerAlicia M Fry

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Citations

Mar 26, 2013·Antiviral Research·Mélanie SamsonGuy Boivin
Sep 22, 2015·Viruses·Timothy C M LiNelson Lee
Dec 15, 2015·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Sebastian GrundOrtwin Adams
Oct 16, 2015·Jundishapur Journal of Microbiology·Nastaran KhodadadAmir Emami
Sep 10, 2013·Antiviral Research·Andrew J BurnhamElena A Govorkova
Jul 8, 2014·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Miguel E Quiñones-MateuMiguel A Martinez
Sep 7, 2016·Virus Research·Lesly Romero-BeltranGuadalupe Ayora-Talavera
Jun 13, 2019·Influenza and Other Respiratory Viruses·Erin N HodgesLarisa V Gubareva
Sep 22, 2017·The Journal of Infectious Diseases·Maki KisoYoshihiro Kawaoka
Sep 21, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Richard J Whitley, Arnold S Monto
Sep 21, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sylvie BehillilNicolas de Prost

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