PMID: 9423886Jan 10, 1998Paper

Analysis of inhibitory epitopes in the Plasmodium falciparum rhoptry protein RAP-1 including identification of a second inhibitory epitope

Infection and Immunity
R F HowardJ Thurman

Abstract

Immune responses to Plasmodium falciparum rhoptry-associated protein 1 (RAP-1), RAP-2, and RAP-3 appear to contribute to protection against infection by this human malarial parasite. This conclusion is suggested by results of monkey immunization trials and of cell culture studies showing antibody-dependent inhibition of erythrocyte invasion. In the present study, splenectomized owl monkeys were infected with P. falciparum in order to monitor anti-RAP-1 antibody production as antiparasite immunity developed. The monkeys responded to a primary infection with the production of antibodies to a fragment of RAP-1 containing amino acids 1 to 294 (RAP-1(1-294)). After drug cure and reinfection, the monkeys had a prolonged prepatent period, indicating they had already developed partial immunity to the parasite. Sera from these animals showed major increases in anti-RAP-1(1-294) antibodies. In contrast, only low levels of antibodies to inhibitory B-cell epitope 1 (iB-1), an inhibitory epitope in RAP-1(1-294) with the sequence N200TLTPLEELYPT211, was observed after the initial parasite infection, and the anti-iB-1 antibodies were not readily boosted upon reinfection. These results suggest that iB-1 is an immunogenic but not immunodominant...Continue Reading

References

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Citations

Feb 13, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Alan F CowmanBrendan S Crabb
Nov 14, 2003·The Journal of Biological Chemistry·Agnieszka E TopolskaRoss L Coppel
Sep 9, 2010·Infection and Immunity·Babita MahajanSanjai Kumar
Feb 27, 2001·Current Opinion in Hematology·C E Chitnis
Sep 1, 2009·Expert Review of Clinical Pharmacology·Athar AlamUday Bandyopadhyay
Sep 13, 2002·Immunology and Cell Biology·Jiraprapa WipasaMichael F Good
Jun 2, 2011·Molecular & Cellular Proteomics : MCP·Angela TrieuDenise L Doolan
Apr 7, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·M Andreína PachecoAnanias A Escalante

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