PMID: 9421110Jan 8, 1998Paper

Analysis of isepamicin in human plasma by radioimmunoassay, microbiologic assay, and high-performance liquid chromatography

Therapeutic Drug Monitoring
C C LinA Nomeir

Abstract

Plasma concentrations of isepamicin, a new aminoglycoside antibiotic, were determined by radioimmunoassay (RIA), microbiological assay (MA), and high-performance liquid chromatography (HPLC) in healthy volunteers after administration of 7.5 mg/kg intramuscular dosages once daily for 10 days. Plasma samples were collected on days 1, 7, and 10. The limit of quantitation (LOQ) was 0.1 microg/ml for HPLC and RIA and 0.5 microg/ml for MA. The HPLC and RIA yielded superimposable plasma concentration-time curves, whereas the plasma concentrations obtained with MA appeared to be 20% to 30% lower. Regression analysis indicated good correlations among the three assays, with coefficients of correlation measuring 0.935 to 0.960 for RIA compared with HPLC, 0.925 to 0.945 for MA compared with HPLC, and 0.920 to 0.945 for RIA compared with MA.

References

Sep 1, 1977·Clinical Pharmacology and Therapeutics·J J Schentag, W J Jusko
Dec 1, 1977·Journal of Pharmacokinetics and Biopharmaceutics·J J SchentagL M Gerbracht
May 1, 1978·Clinical Pharmacology and Therapeutics·J A JahreH C Neu
Apr 1, 1977·Antimicrobial Agents and Chemotherapy·J P Anhalt
Jun 1, 1977·Antimicrobial Agents and Chemotherapy·M L Kornguth, C M Kunin
Dec 1, 1977·Antimicrobial Agents and Chemotherapy·G W PengW L Chiou
Apr 1, 1973·Antimicrobial Agents and Chemotherapy·V K SimonV Malerczy
Apr 1, 1973·Antimicrobial Agents and Chemotherapy·K G NaberP O Madsen
Nov 1, 1983·Clinical Pharmacology and Therapeutics·O L LaskinP S Lietman
Oct 1, 1982·The Journal of Antimicrobial Chemotherapy·J Marples, M D Oates
Oct 1, 1981·Journal of Pharmacokinetics and Biopharmaceutics·M ChungS Symchowicz
Feb 1, 1980·Antimicrobial Agents and Chemotherapy·M ChungS Symchowicz
Apr 1, 1980·Antimicrobial Agents and Chemotherapy·D B HaugheyJ J Schentag
Mar 1, 1995·Antimicrobial Agents and Chemotherapy·K V Clemons, D A Stevens
Dec 1, 1995·Antimicrobial Agents and Chemotherapy·C C LinM Affrime
Oct 1, 1995·Antimicrobial Agents and Chemotherapy·C LinM N Cayen

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Citations

Mar 20, 1998·Journal of Clinical Pharmacology·A A NomeirM N Cayen
Dec 5, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·D A Stead
Dec 7, 2002·Journal of Pharmaceutical and Biomedical Analysis·Ibrahim A Darwish

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