Analysis of KaiA-KaiC protein interactions in the cyano-bacterial circadian clock using hybrid structural methods.

The EMBO Journal
Rekha PattanayekMartin Egli

Abstract

The cyanobacterial circadian clock can be reconstituted in vitro by mixing recombinant KaiA, KaiB and KaiC proteins with ATP, producing KaiC phosphorylation and dephosphorylation cycles that have a regular rhythm with a ca. 24-h period and are temperature-compensated. KaiA and KaiB are modulators of KaiC phosphorylation, whereby KaiB antagonizes KaiA's action. Here, we present a complete crystallographic model of the Synechococcus elongatus KaiC hexamer that includes previously unresolved portions of the C-terminal regions, and a negative-stain electron microscopy study of S. elongatus and Thermosynechococcus elongatus BP-1 KaiA-KaiC complexes. Site-directed mutagenesis in combination with EM reveals that KaiA binds exclusively to the CII half of the KaiC hexamer. The EM-based model of the KaiA-KaiC complex reveals protein-protein interactions at two sites: the known interaction of the flexible C-terminal KaiC peptide with KaiA, and a second postulated interaction between the apical region of KaiA and the ATP binding cleft on KaiC. This model brings KaiA mutation sites that alter clock period or abolish rhythmicity into contact with KaiC and suggests how KaiA might regulate KaiC phosphorylation.

References

Jan 1, 1996·Journal of Structural Biology·M van HeelM Schatz
Dec 22, 1999·Journal of Structural Biology·S J LudtkeW Chiu
Oct 23, 2002·Proceedings of the National Academy of Sciences of the United States of America·Hideo IwasakiTakao Kondo
Nov 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·Stanly B WilliamsAndy C LiWang
Nov 21, 2002·The Journal of Biological Chemistry·Hakuto KageyamaHideo Iwasaki
Dec 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tetsuya MoriCarl H Johnson
Mar 8, 2003·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Fumio HayashiMasahiro Ishiura
Jan 8, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yoichi NakahiraTakao Kondo
Jan 30, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ioannis VakonakisAndy C LiWang
Mar 9, 2004·Biochemical and Biophysical Research Communications·Fumio HayashiMasahiro Ishiura
Mar 10, 2004·The Journal of Biological Chemistry·Sheng YeJames C Sacchettini
Jun 1, 2004·Nature Structural & Molecular Biology·Tatsuya UzumakiMasahiro Ishiura
Jun 29, 2004·Nature Structural & Molecular Biology·Carl H Johnson, Martin Egli
Jul 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ioannis Vakonakis, Andy C LiWang
Sep 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yao XuCarl Hirschie Johnson
Sep 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Taeko NishiwakiTakao Kondo
Sep 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Susan S Golden
Sep 21, 2004·The Journal of Biological Chemistry·Fumio HayashiMasahiro Ishiura
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·T Alwyn Jones

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Citations

Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yong-Ick KimAndy LiWang
Aug 4, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ximing QinCarl Hirschie Johnson
Jul 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yong-Gang ChangAndy LiWang
Oct 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yong-Ick KimSusan S Golden
Apr 19, 2008·Cold Spring Harbor Symposia on Quantitative Biology·C H Johnson
Apr 19, 2008·Cold Spring Harbor Symposia on Quantitative Biology·T Kondo
Nov 1, 2008·Science·Carl Hirschie JohnsonPhoebe L Stewart
May 29, 2007·Journal of Bacteriology·Shane B ClaggettBrian D Cain
Mar 29, 2007·PLoS Biology·Tetsuya MoriCarl Hirschie Johnson
Jul 26, 2006·PLoS Computational Biology·Arun MehraPeter Ruoff
May 4, 2007·PloS One·Mitsumasa YodaMasaki Sasai
Jun 7, 2014·Annual Review of Biochemistry·Carl Hirschie Johnson, Martin Egli
Apr 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jeroen S van ZonPieter Rein ten Wolde
Jan 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Joost SnijderAlbert J R Heck
Feb 22, 2011·Annual Review of Biophysics·Carl Hirschie JohnsonMartin Egli
Jan 26, 2012·Cellular and Molecular Life Sciences : CMLS·Shuji Akiyama
Oct 12, 2010·Current Opinion in Genetics & Development·Guogang DongSusan S Golden
Nov 17, 2009·FEBS Letters·Joseph S Markson, Erin K O'Shea
Nov 6, 2008·Current Opinion in Microbiology·Guogang Dong, Susan S Golden
Mar 14, 2007·Molecular Systems Biology·Sébastien ClodongMarkus Kollmann
Mar 28, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Reiko MurakamiMasahiro Ishiura
May 27, 2008·Biophysical Journal·Kohei EguchiMasaki Sasai
Feb 11, 2014·Biophysical Journal·Jane S Richardson, David C Richardson
Feb 8, 2013·Cell Cycle·Rekha PattanayekMartin Egli
Sep 13, 2008·Current Biology : CB·Carl Hirschie JohnsonYao Xu
Mar 18, 2008·Molecular Cell·Shuji AkiyamaYuichiro Maéda
Jun 2, 2010·Biophysical Journal·Tetsuro NagaiMasaki Sasai
Mar 16, 2017·PLoS Computational Biology·Joris PaijmansPieter Rein Ten Wolde
May 19, 2017·Proceedings of the National Academy of Sciences of the United States of America·Yoriko MurayamaHiroshi Ito
Jun 5, 2019·Life Science Alliance·Yasuhiro YunokiKoichi Kato
May 13, 2014·Acta Crystallographica. Section D, Biological Crystallography·Rekha PattanayekMartin Egli
Dec 14, 2017·Scientific Reports·Sumita DasMasaki Sasai
Jun 18, 2014·The Journal of Biological Chemistry·Martin Egli
Nov 18, 2018·Proceedings of the National Academy of Sciences of the United States of America·Lu HongAaron R Dinner

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