Analysis of Ki-ras gene mutations within the same tumor using a single tumor crypt in colorectal carcinomas

Journal of Gastroenterology
Motohiro IshiiShin-Ichi Nakamura

Abstract

The point mutations occurring in codons 12 and 13 of the Ki- ras gene are useful genetic markers to identify intratumoral heterogeneity. A single tumor crypt, which consists of monoclonal cells, can be obtained using the crypt isolation method. Ki- ras gene mutations have been examined using the crypt isolation method to determine whether multiclonarity is present within the same tumor. Ki- ras gene mutations were analyzed using a crypt isolation technique coupled with polymerase chain reaction and direct sequencing in 21 sporadic colorectal carcinomas. The specimens were divided into two groups: a representative sample, which was composed of more than 50 tumor crypts, and a single tumor crypt sample. The latter consisted of 10 single tumor crypts, which were obtained from the same tumor separately. Ki- ras gene mutations were found in 11 of 21 representative samples and in 12 of 21 single tumor crypt samples. In the 11 samples with Ki- ras mutation, Ki- ras mutations were also found in most single tumor crypts. Among the 12 base substitutions found, G:C to A:T transitions were the most commonly observed. There were no differences between the two samples in the types of Ki- ras mutations found. One Ki- ras mutation that was not...Continue Reading

Citations

May 17, 2011·International Journal of Colorectal Disease·Satu OltedalOddmund Nordgård
May 4, 2011·Pathology, Research and Practice·Hans BösmüllerFalko Fend
Jun 13, 2009·International Journal of Cancer. Journal International Du Cancer·Gisela KellerAurel Perren
Sep 22, 2012·The American Journal of Pathology·Eiichiro YamamotoMinoru Toyota
Oct 26, 2016·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Takayuki NagasawaToru Sugiyama
May 16, 2006·Journal of Gastroenterology·Tetsuji TakayamaYoshiro Niitsu
Nov 1, 2016·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Takayuki NagasawaToru Sugiyama
Jan 28, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kenji KawadaYoshiharu Sakai
May 4, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yoon Pin Lim

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