Analysis of kinesin motor function at budding yeast kinetochores

The Journal of Cell Biology
Jessica D Tytell, Peter K Sorger

Abstract

Accurate chromosome segregation during mitosis requires biorientation of sister chromatids on the microtubules (MT) of the mitotic spindle. Chromosome-MT binding is mediated by kinetochores, which are multiprotein structures that assemble on centromeric (CEN) DNA. The simple CENs of budding yeast are among the best understood, but the roles of kinesin motor proteins at yeast kinetochores have yet to be determined, despite evidence of their importance in higher eukaryotes. We show that all four nuclear kinesins in Saccharomyces cerevisiae localize to kinetochores and function in three distinct processes. Kip1p and Cin8p, which are kinesin-5/BimC family members, cluster kinetochores into their characteristic bilobed metaphase configuration. Kip3p, a kinesin-8,-13/KinI kinesin, synchronizes poleward kinetochore movement during anaphase A. The kinesin-14 motor Kar3p appears to function at the subset of kinetochores that become detached from spindle MTs. These data demonstrate roles for structurally diverse motors in the complex processes of chromosome segregation and reveal important similarities and intriguing differences between higher and lower eukaryotes.

References

Jul 1, 1992·The Journal of Cell Biology·D M RoofM D Rose
Aug 9, 1991·Cell·R Li, A W Murray
Jun 1, 1995·The Journal of Cell Biology·M WineyJ R McIntosh
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·K Middleton, J Carbon
May 1, 1993·The Journal of Cell Biology·P Y Goh, J V Kilmartin
Jul 25, 1997·Science·A F StraightA W Murray
Jul 31, 1999·The Journal of Cell Biology·X ZengW S Saunders
Sep 25, 1999·The Journal of Biological Chemistry·D M Gordon, D M Roof
Mar 21, 2000·Biochimica Et Biophysica Acta·E R Hildebrandt, M A Hoyt
Jan 9, 2001·Nature Cell Biology·T J Mitchison, E D Salmon
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·D M Gordon, D M Roof
Feb 28, 2002·Journal of Molecular Biology·Hanspeter NiederstrasserEva Nogales
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Andrew D McAinshPeter K Sorger
Dec 6, 2003·Journal of Cell Science·Elise M Dagenbach, Sharyn A Endow
Feb 11, 2004·The Journal of Cell Biology·Emily S GillettPeter K Sorger
Sep 29, 2004·Trends in Cell Biology·Ayana Moore, Linda Wordeman
Oct 29, 2004·Journal of Cell Science·Helder MaiatoWilliam C Earnshaw
Nov 9, 2004·Current Biology : CB·Chad G PearsonKerry Bloom
Jan 11, 2005·Nature Structural & Molecular Biology·J J L MirandaStephen C Harrison
Jan 22, 2005·Current Opinion in Cell Biology·Susan L Kline-SmithArshad Desai
Apr 23, 2005·Nature·Kozo TanakaTomoyuki U Tanaka
May 6, 2005·Nature·Lukas C KapiteinChristoph F Schmidt
Aug 9, 2005·Current Biology : CB·Lisa R SproulSusan P Gilbert

❮ Previous
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Citations

Feb 1, 2012·Chromosoma·Bungo Akiyoshi, Sue Biggins
Sep 2, 2006·Nature Cell Biology·Claire E Walczak
Aug 3, 2013·Nature Cell Biology·Johanna Roostalu, Thomas Surrey
Dec 22, 2007·Nature Reviews. Molecular Cell Biology·Iain M Cheeseman, Arshad Desai
Oct 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Hery RatsimaDamien D'Amours
Nov 6, 2008·The Journal of Biological Chemistry·Ken'ya FurutaYoko Y Toyoshima
Nov 6, 2013·The Journal of Cell Biology·Andrew D StephensKerry Bloom
May 18, 2007·Molecular Biology of the Cell·Lina MaVivien Measday
Apr 6, 2007·Molecular Biology of the Cell·Ekaterina L GrishchukJ Richard McIntosh
Apr 27, 2007·Molecular Biology of the Cell·J J L MirandaStephen C Harrison
Jul 20, 2007·Molecular Biology of the Cell·Jonathan WongTony Hazbun
Feb 26, 2011·Science·Johanna RoostaluThomas Surrey
Mar 17, 2007·Annual Review of Biochemistry·Stefan WestermannGeorjana Barnes
Mar 5, 2014·Proceedings of the National Academy of Sciences of the United States of America·Tomohiro MikiGohta Goshima
Jul 8, 2010·Annual Review of Cell and Developmental Biology·Gohta Goshima, Jonathan M Scholey
Jun 27, 2014·Chromosoma·Francesca Malvezzi, Stefan Westermann
Feb 26, 2013·FEBS Letters·Taras Makhnevych, Walid A Houry
Aug 22, 2012·The Journal of Cell Biology·Taras MakhnevychWalid A Houry
Apr 13, 2011·The Journal of Cell Biology·Daniel K RozelleKenneth B Kaplan
Sep 12, 2015·Molecular Biology of the Cell·Kelsey M McCoyDavid J Odde
Jun 3, 2008·Trends in Cell Biology·Melissa K GardnerKerry Bloom
Jan 10, 2008·Current Opinion in Cell Biology·Tomoyuki U Tanaka, Arshad Desai
Dec 23, 2006·Current Opinion in Cell Biology·Jonathon Howard, Anthony A Hyman
Nov 30, 2006·Trends in Microbiology·Gero Steinberg
Mar 19, 2009·Molecular Systems Biology·Christian TischerMarileen Dogterom
Oct 19, 2006·Current Opinion in Cell Biology·Melissa K Gardner, David J Odde
Jul 4, 2012·Bioarchitecture·Christina ThiedeChristoph F Schmidt
Apr 7, 2007·Current Biology : CB·David C Bouck, Kerry Bloom
May 2, 2007·Current Biology : CB·Jason Stumpff, Linda Wordeman
Apr 10, 2008·Current Biology : CB·Marcel E Janson, Phong T Tran
Aug 31, 2006·Biophysical Journal·Khuloud JaqamanGaudenz Danuser
Mar 5, 2014·Biochemical and Biophysical Research Communications·Masaki Edamatsu
Nov 5, 2016·Molecular Biology of the Cell·Zachary R GergelyMeredith D Betterton

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Methods Mentioned

BETA
immunoprecipitation
ChIP
PCR

Software Mentioned

Machine Vision

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