Analysis of ligand binding by bioaffinity mass spectrometry

Clinica Chimica Acta; International Journal of Clinical Chemistry
Yusheng ZhuSaeed A Jortani

Abstract

Ligand binding is commonly analyzed using various immunoassays that are generally time-consuming and some may require secondary antibodies or gel electrophoresis which are also time-consuming and sometimes subjective. We introduced various examples for a more rapid approach using pre-activated surface chips which are analyzed by surface enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF MS). Specific applications presented in this study include immobilization of antigen, antibody or oligo DNA on pre-activated chips with subsequent identification of the binding antibodies, antigens or DNA binding proteins to demonstrate the universal utility of this novel approach. BSA-digoxin conjugate (BSA-Dig), anti-digoxin antibody, anti-urinary trypsin inhibitor (uTi) antibody, or a double stranded oligo nucleotide based on the nucleotide sequence between -91 and -10 of the human CYP 450 2E1 promoter were immobilized on the Ciphergen pre-activated surface chips. Anti-digoxin antibody, BSA-digoxin conjugate, uTi, and CYP450 2E1 promoter binding protein were captured on the chip and identified by SELDI-TOF MS. A protein with 141kDa was identified from anti-digoxin serum using BSA-Dig chips. This binding was compe...Continue Reading

References

Jun 1, 1991·Hypertension·D W HarrisD W DuCharme
Sep 29, 1999·Neuroreport·E R FrearsB M Austen
Nov 30, 2000·Biochemical and Biophysical Research Communications·S S D'CostaM D Boyle
Mar 22, 2001·Pharmacogenomics·S R WeinbergerM Pawlak
Feb 1, 2002·The Journal of Biological Chemistry·Yousef G AmaarSubburaman Mohan
Nov 16, 2004·Journal of Clinical Laboratory Analysis·Saeed A JortaniRoland Valdes

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Citations

Mar 23, 2007·Biomolecular Engineering·Ronny FalkSophia Hober
Aug 1, 2007·Expert Opinion on Drug Discovery·Geoff A Holdgate
May 3, 2007·Journal of Mass Spectrometry : JMS

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