Analysis of Light- and Carbon-Specific Transcriptomes Implicates a Class of G-Protein-Coupled Receptors in Cellulose Sensing

MSphere
Eva StapplerMonika Schmoll

Abstract

In fungi, most metabolic processes are subject to regulation by light. Trichoderma reesei is adapted to degradation of plant cell walls and regulates production of the required enzymes in a manner dependent on the nutrient source and the light status. Here we investigated the interrelated relevance of two regulation levels of the transcriptome of T. reesei: light regulation and carbon source-dependent control. We show that the carbon source (cellulose, lactose, sophorose, glucose, or glycerol) is the major source of variation, with light having a modulating effect on transcript regulation. A total of 907 genes were regulated under cellulase-inducing conditions in light, and 947 genes were regulated in darkness, with 530 genes overlapping (1,324 in total). Only 218 of the 1,324 induction-specific genes were independent of light and not regulated by the BLR1, BLR2, and ENV1 photoreceptors. Analysis of the genomic distribution of genes regulated by light upon growth on cellulose revealed considerable overlap of light-regulated clusters with induction-specific clusters and carbohydrate-active enzyme (CAZyme) clusters. Further, we found evidence for the operation of a sensing mechanism for solid cellulosic substrates, with regulatio...Continue Reading

References

Apr 1, 1997·Applied and Environmental Microbiology·M IlménM E Penttilä
Jun 15, 1997·FEMS Microbiology Letters·G J Ruijter, J Visser
May 13, 1999·The Journal of Biological Chemistry·G Berrocal-TitoA Herrera-Estrella
Jun 6, 2003·The Journal of Biological Chemistry·Pamela K ForemanMichael Ward
Feb 1, 1962·Journal of Bacteriology·M MANDELSE T REESE
Aug 17, 2005·FEMS Microbiology Reviews·Nina AroMerja Penttilä
Oct 18, 2006·BMC Bioinformatics·Alessandro CoppeStefania Bortoluzzi
Mar 27, 2007·PLoS Computational Biology·Regina BrockmannThomas Wilhelm
May 18, 2007·Annual Review of Microbiology·Liande LiKatherine A Borkovich
Aug 7, 2007·FEBS Letters·Xin-Liang LiPeter Biely
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Verena SeidlMonika Schmoll
Nov 17, 2009·Applied Microbiology and Biotechnology·Doris Tisch, Monika Schmoll
Feb 11, 2010·Fungal Genetics and Biology : FG & B·Francisco CastellanosEdgardo Ulises Esquivel-Naranjo
May 15, 2010·Fungal Genetics and Biology : FG & B·Monika SchmollAlfredo Herrera-Estrella
Jun 11, 2010·Annual Review of Microbiology·Julio Rodriguez-RomeroReinhard Fischer
Dec 22, 2011·BMC Genomics·Mikko ArvasMarkku Saloheimo
Jan 4, 2012·Biotechnology for Biofuels·André SchusterMonika Schmoll
Jan 31, 2012·Applied and Environmental Microbiology·André SchusterMonika Schmoll
May 5, 2012·Molecular Microbiology·Bernhard SeibothChristian P Kubicek
Aug 14, 2012·Fungal Genetics and Biology : FG & B·Christian SeibelMonika Schmoll
Jan 10, 2013·Applied Microbiology and Biotechnology·Levente KaraffaChristian P Kubicek
Jan 24, 2013·Biotechnology for Biofuels·Elizabeth A Znameroski, N Louise Glass

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Citations

Mar 29, 2018·Nature Microbiology·Neil Andrew BrownGustavo Henrique Goldman
Jan 30, 2018·Frontiers in Microbiology·Eva StapplerMonika Schmoll
Jul 17, 2019·Molecular Microbiology·Yanli CaoWeifeng Liu
Sep 19, 2019·Fungal biology and biotechnology·Wolfgang HinterdoblerMonika Schmoll
May 2, 2018·Fungal biology and biotechnology·Monika Schmoll
Jul 31, 2019·Scientific Reports·Aroa Rodriguez-Iglesias, Monika Schmoll
May 23, 2018·Fungal biology and biotechnology·Christoph DattenböckMonika Schmoll
Dec 1, 2020·Frontiers in Bioengineering and Biotechnology·Wolfgang HinterdoblerMonika Schmoll
Jul 21, 2021·Scientific Reports·Sami HavukainenChristopher P Landowski
Sep 10, 2021·Microbial Cell Factories·Sami HavukainenChristopher P Landowski

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Datasets Mentioned

BETA
GSE36448
GSE81365

Methods Mentioned

BETA
transglycosylation
GTPase
glycosylation
PCR
reverse transcription-PCR
electrophoresis
Chip

Software Mentioned

Partek Genomics suite
MIPS Functional Catalogue
HCE3
PSPP
REEF

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