Analysis of lipid adsorption on nanoparticles by nanoflow liquid chromatography-tandem mass spectrometry

Analytical and Bioanalytical Chemistry
Ju Yong LeeWenwan Zhong

Abstract

Nanoparticles (NPs) tend to adsorb matrix molecules like proteins and lipids incubated with biological fluids, forming a biological corona. While the formation and functions of protein corona have been studied extensively, little attention has been paid to lipid adsorption on NPs. However, lipids are also abundantly present in biological fluids and play important roles in processes like cell signaling and angiogenesis. Therefore, in this study, we established the analytical procedure for study of lipid adsorption on three different types of NPs in two matrices: human serum and heavy cream, using nanoflow liquid chromatography-mass spectrometry (nanoflowLC-MS). Serum was chosen to represent the common environment the NPs would be present once entering human body, and heavy cream was the representative food matrix NPs may be added to improve the color or taste. Steps of liquid-liquid extraction were established and optimized to achieve maximum recovery of the adsorbed, standard lipids from the NPs. Then, the LC-MS/MS method was developed to attain base-line separation of the standard lipids that represent the major lipid classes. At last, the lipid adsorption profiles of the three NPs were compared. We found that the lipid adsorp...Continue Reading

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Citations

Feb 14, 2019·Analytical and Bioanalytical Chemistry·Anna Laura CapriottiSusy Piovesana
Aug 23, 2018·Analytical and Bioanalytical Chemistry·Lisa Holland, Wenwan Zhong
Mar 7, 2020·Environmental Science. Nano·Glen M DeLoidPhilip Demokritou
Aug 28, 2020·Nanoscale Horizons : the Home for Rapid Reports of Exceptional Significance in Nanoscience and Nanotechnolgy·Lois GardnerMarilena Hadjidemetriou
Jun 13, 2021·Nature Nanotechnology·Korin E WheelerIseult Lynch
Sep 15, 2021·Journal of Extracellular Vesicles·Eszter Á TóthEdit I Buzás
Nov 18, 2021·The Analyst·Takayuki KawaiKoji Otsuka
Oct 7, 2021·Journal of Food Protection·Jefferson de Oliveira MalliaVasilis Valdramidis

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