Oct 1, 1989

Analysis of lipopolysaccharide antigens of Treponema hyodysenteriae

Epidemiology and Infection
D J HampsonB Combs

Abstract

Lipopolysaccharide (LPS) extracts obtained from Treponema hyodysenteriae of serogroups A, B, D and E, and from T. innocens were examined by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), silver-staining, and immunoblotting with hyperimmune rabbit sera. All organisms possessed multiple LPS bands, but their position and number differed. Immunoblotting of LPS with grouping sera identified three or four major antigenic LPS components in the 10-42 kDa range in all organisms: these components were largely specific to each type-organism of a serogroup, and presumably represented group antigens. Although some minor cross-reactivity occurred between LPS from organisms in the different groups, this was insufficient to merit changes to the current LPS serogrouping system for T. hyodysenteriae. Besides this LPS 'complex', other higher-molecular-weight material which appeared to be a common component of the treponemes examined was present in low concentrations. Organisms with different serotypes within a serogroup apparently possessed common LPS bands, but also had unique LPS bands which may account for their serotype specificity. One 'untypable' organism lacked group-specific LPS and was thought to be a mutant of a group B organism. Th...Continue Reading

  • References8
  • Citations3

References

  • References8
  • Citations3

Citations

Mentioned in this Paper

Pathogenic Aspects
Immunoblotting, Reverse
Pathogenesis
Brachyspira hyodysenteriae (bacteria)
SDS-PAGE
Complex (molecular entity)
Cross Reactions
Brachyspira innocens
Phosphoglycerate Dehydrogenase Activity
Immunoblotting

About this Paper

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