PMID: 29907Sep 1, 1978

Analysis of lorazepam and its glucuronide metabolite by electron-capture gas--liquid chromatography. Use in pharmacokinetic studies of lorazepam

Journal of Chromatography
D J GreenblattR I Shader


This paper describes a rapid and sensitive method for analysis of lorazepam and its glucuronide metabolite in plasma and urine following therapeutic doses of lorazepam in humans. After addition of the structurally related benzodiazepine derivative, oxazepam, as the internal standard, 1-ml samples of plasma or urine are extracted twice at neutral pH with benzene (containing 1.5% isoamyl alcohol). The combined extracts are evaporated to dryness, reconstituted, and subjected to gas chromatographic analysis using a 3% OV-17 column and an electron-capture detector. Lorazepam glucuronide in urine is similarly analyzed following enzymatic cleavage with Glusulase. The sensitivity limits are 1--3 ng of analyzed following enzymatic cleavage with Glusulase. The sensitivity limits are 1--3 ng of lorazepam per ml of original sample, and the variability of identical samples is 5% or less. The applicability of the method to pharmacokinetic studies of lorazepam is demonstrated.


May 1, 1997·Journal of Clinical Pharmacology·E E SamaraJ H Cavanaugh
Jan 1, 1983·Psychopharmacology·R G ListerD J Greenblatt
Jan 1, 1979·Psychopharmacology·M D AllenJ D Arnold
Jan 1, 1987·European Journal of Clinical Pharmacology·H R OchsR I Shader
Jan 1, 1988·European Journal of Clinical Pharmacology·S R BareggiS Smirne
Aug 16, 1984·Klinische Wochenschrift·H R OchsU Klehr
Aug 17, 1981·Klinische Wochenschrift·H R OchsH Otten
Jul 1, 1986·European Journal of Drug Metabolism and Pharmacokinetics·S R BareggiL Zecca
Aug 29, 1989·European Journal of Pharmacology·C A SannerudR R Griffiths
Jun 23, 2001·Pharmacology, Biochemistry, and Behavior·Jeanne M FaheyDavid J Greenblatt
Nov 7, 2001·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J L DorneA G Renwick
Jun 22, 1999·Schizophrenia Research·G NorthoffBernhard Bogerts
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·E MatthewS M Paul
Aug 31, 2000·Critical Care Medicine·D J GreenblattR I Shader
Apr 4, 1981·British Medical Journal·A G WhitelawI R McFadyen
Sep 10, 2014·Progress in Neuro-psychopharmacology & Biological Psychiatry·Nunzio PomaraJohn J Sidtis
Apr 21, 2005·Psychiatry Research·Raiko BarkG Northoff
Sep 29, 2004·Forensic Science International : Synergy·Pascal KintzBertrand Ludes
Sep 23, 2006·Brain Research·Jeanne M FaheyDavid J Greenblatt
May 1, 1981·Scandinavian Journal of Clinical and Laboratory Investigation·J Lund
Dec 25, 2003·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Nunzio PomaraCharles B Nemeroff
Aug 1, 1990·Biopharmaceutics & Drug Disposition·A Locniskar, D J Greenblatt
Jun 1, 1982·Acta Anaesthesiologica Scandinavica·K KorttilaG Abbondati
Dec 1, 1983·Perceptual and Motor Skills·R Berchou, R I Block
Jan 1, 1987·Psychopharmacology·E H EllinwoodT D Bjornsson
Oct 8, 1997·Journal of Clinical Psychopharmacology·R I Shader, D J Greenblatt
Apr 1, 1996·Therapeutic Drug Monitoring·P G AgbuyaA D Morris
Dec 14, 1978·The New England Journal of Medicine·D J Greenblatt, R I Shader


Jun 8, 1976·Archives of Toxicology·G de GrootH H Lemmens
Aug 1, 1977·Journal of Clinical Pharmacology·D J GreenblattH W Ruelius
Jan 1, 1976·Clinical Pharmacokinetics·D J GreenblattJ Koch-Weser
Sep 1, 1973·Analytical Chemistry·A FrigerioG Belvedere
Jul 8, 1971·Journal of Chromatography·A ForgioneG C Jommi

Related Concepts

Lorazepam glucuronide
Anti-Anxiety Effect
Cytokinesis of the Fertilized Ovum
Isopentyl alcohol
Glucuronic Acids
Benzodiazepine [EPC]

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.