PMID: 29907Sep 1, 1978

Analysis of lorazepam and its glucuronide metabolite by electron-capture gas--liquid chromatography. Use in pharmacokinetic studies of lorazepam

Journal of Chromatography
D J GreenblattR I Shader

Abstract

This paper describes a rapid and sensitive method for analysis of lorazepam and its glucuronide metabolite in plasma and urine following therapeutic doses of lorazepam in humans. After addition of the structurally related benzodiazepine derivative, oxazepam, as the internal standard, 1-ml samples of plasma or urine are extracted twice at neutral pH with benzene (containing 1.5% isoamyl alcohol). The combined extracts are evaporated to dryness, reconstituted, and subjected to gas chromatographic analysis using a 3% OV-17 column and an electron-capture detector. Lorazepam glucuronide in urine is similarly analyzed following enzymatic cleavage with Glusulase. The sensitivity limits are 1--3 ng of analyzed following enzymatic cleavage with Glusulase. The sensitivity limits are 1--3 ng of lorazepam per ml of original sample, and the variability of identical samples is 5% or less. The applicability of the method to pharmacokinetic studies of lorazepam is demonstrated.

References

May 1, 1997·Journal of Clinical Pharmacology·E E SamaraJ H Cavanaugh
Jan 1, 1983·Psychopharmacology·R G ListerD J Greenblatt
Jan 1, 1979·Psychopharmacology·M D AllenJ D Arnold
Jan 1, 1987·European Journal of Clinical Pharmacology·H R OchsR I Shader
Jan 1, 1988·European Journal of Clinical Pharmacology·S R BareggiS Smirne
Aug 16, 1984·Klinische Wochenschrift·H R OchsU Klehr
Aug 17, 1981·Klinische Wochenschrift·H R OchsH Otten
Jul 1, 1986·European Journal of Drug Metabolism and Pharmacokinetics·S R BareggiL Zecca
Aug 29, 1989·European Journal of Pharmacology·C A SannerudR R Griffiths
Jun 23, 2001·Pharmacology, Biochemistry, and Behavior·Jeanne M FaheyDavid J Greenblatt
Nov 7, 2001·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J L DorneA G Renwick
Jun 22, 1999·Schizophrenia Research·G NorthoffBernhard Bogerts
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·E MatthewS M Paul
Aug 31, 2000·Critical Care Medicine·D J GreenblattR I Shader
Apr 4, 1981·British Medical Journal·A G WhitelawI R McFadyen
Sep 10, 2014·Progress in Neuro-psychopharmacology & Biological Psychiatry·Nunzio PomaraJohn J Sidtis
Apr 21, 2005·Psychiatry Research·Raiko BarkG Northoff
Sep 29, 2004·Forensic Science International : Synergy·Pascal KintzBertrand Ludes
Sep 23, 2006·Brain Research·Jeanne M FaheyDavid J Greenblatt
May 1, 1981·Scandinavian Journal of Clinical and Laboratory Investigation·J Lund
Dec 25, 2003·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Nunzio PomaraCharles B Nemeroff
Aug 1, 1990·Biopharmaceutics & Drug Disposition·A Locniskar, D J Greenblatt
Jun 1, 1982·Acta Anaesthesiologica Scandinavica·K KorttilaG Abbondati
Dec 1, 1983·Perceptual and Motor Skills·R Berchou, R I Block
Jan 1, 1987·Psychopharmacology·E H EllinwoodT D Bjornsson
Oct 8, 1997·Journal of Clinical Psychopharmacology·R I Shader, D J Greenblatt
Apr 1, 1996·Therapeutic Drug Monitoring·P G AgbuyaA D Morris
Dec 14, 1978·The New England Journal of Medicine·D J Greenblatt, R I Shader

Citations

Jun 8, 1976·Archives of Toxicology·G de GrootH H Lemmens
Aug 1, 1977·Journal of Clinical Pharmacology·D J GreenblattH W Ruelius
Jan 1, 1976·Clinical Pharmacokinetics·D J GreenblattJ Koch-Weser
Sep 1, 1973·Analytical Chemistry·A FrigerioG Belvedere
Jul 8, 1971·Journal of Chromatography·A ForgioneG C Jommi

Related Concepts

Urine
Lorazepam glucuronide
Anti-Anxiety Effect
Cytokinesis of the Fertilized Ovum
Benzene
Lorazepam
Isopentyl alcohol
Glucuronic Acids
Benzodiazepine [EPC]
Glusulase

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