PMID: 9430536Jan 16, 1998Paper

Analysis of malaria parasite RNA from decade-old Giemsa-stained blood smears and dried mosquitoes

The American Journal of Tropical Medicine and Hygiene
J LiT F McCutchan

Abstract

We have analyzed RNA isolated from recently prepared and historically preserved slides containing smears of Plasmodium-infected blood. We found that slides preserved as long as 20 years can yield RNA that is a suitable template for polymerase chain reaction (PCR) amplification. Mosquitoes that have been stored for years under ambient temperature can also be used as an RNA source. The RNA amplification from slide-derived material is shown to be dependent upon the addition of reverse transcriptase and the resultant products are specific to the developmental state of the parasite. Amplification of ribosomal RNA with primers conserved for Plasmodium and hybridization with species-specific probes provide a general, unbiased method for species determination. Messenger RNA transcripts from slides also appear to serve as templates. The procedure may add complementary information to that derived from microscopic examination of Giemsa-stained blood smears including species identification, variant antigen identification and drug resistance status.

Citations

Sep 14, 2004·Revista Do Instituto De Medicina Tropical De São Paulo·Silvia Maria Di SantiMarcos Boulos
Dec 4, 2002·Tropical Medicine & International Health : TM & IH·Brian Greenwood
Aug 20, 2019·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·JustinJ S WilcoxHope Hollocher

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