Analysis of membrane-protein complexes of the marine sulfate reducer Desulfobacula toluolica Tol2 by 1D blue native-PAGE complexome profiling and 2D blue native-/SDS-PAGE

Proteomics
Lars WöhlbrandRalf Rabus

Abstract

Sulfate-reducing bacteria (SRB) obtain energy from cytoplasmic reduction of sulfate to sulfide involving APS-reductase (AprAB) and dissimilatory sulfite reductase (DsrAB). These enzymes are predicted to obtain electrons from membrane redox complexes, i.e. the quinone-interacting membrane-bound oxidoreductase (QmoABC) and DsrMKJOP complexes. In addition to these conserved complexes, the genomes of SRB encode a large number of other (predicted) membrane redox complexes, the function and actual formation of which is unknown. This study reports the establishment of 1D Blue Native-PAGE complexome profiling and 2D BN-/SDS-PAGE for analysis of the membrane protein complexome of the marine sulfate reducer Desulfobacula toluolica Tol2. Analysis of normalized score profiles of >800 proteins in combination with hierarchical clustering and identification of 2D BN-/SDS-PAGE separated spots demonstrated separation of membrane complexes in their native form, e.g. ATP synthase. In addition to the QmoABC and DsrMKJOP complexes, other complexes were detected that constitute the basic membrane complexome of D. toluolica Tol2, e.g. transport proteins (e.g. sodium/sulfate symporters) or redox complexes involved in Na(+) -based bioenergetics (RnfABC...Continue Reading

References

Apr 1, 1992·European Journal of Biochemistry·A J PierikW R Hagen
May 1, 1993·Applied and Environmental Microbiology·R RabusF Widdel
Jan 1, 1996·Annual Review of Microbiology·G Deckers-Hebestreit, K Altendorf
Apr 25, 2000·The EMBO Journal·H Schägger, K Pfeiffer
Jun 26, 2001·Research in Microbiology·M A Kertesz
Aug 9, 2003·Biochimica Et Biophysica Acta·Ricardo H PiresInês A C Pereira
Oct 13, 2005·Proteomics·Ilka Wittig, Hermann Schägger
Apr 5, 2007·Nature Protocols·Ilka WittigHermann Schägger
Apr 25, 2007·IUBMB Life·Rita CipollonePaolo Visca
Apr 2, 2008·Annals of the New York Academy of Sciences·Volker MüllerSabrina Dilling
Sep 4, 2008·Proteomics·Ilka Wittig, Hermann Schägger
Oct 17, 2009·Proteomics·Ilka Wittig, Hermann Schägger
Feb 23, 2010·Microbiological Research·Jiri DreslerJiri Stulik
Feb 23, 2010·Molecular & Cellular Proteomics : MCP·Ilka WittigHermann Schägger
Jul 13, 2011·Frontiers in Microbiology·Inês A Cardoso PereiraSofia Santos Venceslau
Apr 25, 2012·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Sofia Marques da SilvaInês A Cardoso Pereira
May 29, 2012·Frontiers in Plant Science·Michael Senkler, Hans-Peter Braun
Sep 18, 2012·Biochimica Et Biophysica Acta·Fabian GreinInês A C Pereira

❮ Previous
Next ❯

Citations

Jul 28, 2016·Biochimica Et Biophysica Acta·Naomi M de AlmeidaBoran Kartal
Sep 8, 2016·Expert Review of Proteomics·Elena L RudashevskayaStavroula Markoutsa
Dec 13, 2016·The Plant Journal : for Cell and Molecular Biology·Jennifer SenklerHans-Peter Braun
Aug 30, 2017·Journal of Molecular Microbiology and Biotechnology·Lars WöhlbrandChristoph Feenders
Mar 20, 2018·Frontiers in Microbiology·Angeliki MarietouKasper U Kjeldsen
Jan 22, 2020·Journal of Basic Microbiology·Erhard RhielRalf Rabus
Jun 28, 2018·Frontiers in Microbiology·Katja SeidelLorenz Adrian
Mar 17, 2021·Biochimica Et Biophysica Acta. Bioenergetics·Joeri van StrienMartijn A Huynen
May 21, 2021·Biochimica Et Biophysica Acta. Bioenergetics·Sergio Guerrero-CastilloSøren W Gersting
Jul 22, 2021·The ISME Journal·Angeliki MarietouBo Barker Jørgensen
Aug 8, 2021·International Journal of Molecular Sciences·Ilka Wittig, Pedro Felipe Malacarne

❮ Previous
Next ❯

Related Concepts

Related Feeds

ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.

Autoimmune Polyendocrinopathies

Autoimmune polyendocrinopathies, also called polyglandular autoimmune syndromes (PGASs), or polyendocrine autoimmune syndromes(PASs), are a heterogeneous group of rare diseases characterized by autoimmune activity against more than one endocrine organ, although non-endocrine organs can be affected. Discover the latest research on autoimmune polyendocrinopathies here.

Autoimmune Polyendocrine Syndromes

This feed focuses on a rare genetic condition called Autoimmune Polyendocrine Syndromes, which are characterized by autoantibodies against multiple endocrine organs. This can lead to Type I Diabetes.