PMID: 9421108Jan 8, 1998Paper

Analysis of methylated 6-mercaptopurine metabolites in human red blood cells: comparison of two methods

Therapeutic Drug Monitoring
T GiverhaugJ Aarbakke

Abstract

Blood samples from 34 recipients of kidney transplants who were on multidrug therapy including azathioprine were analyzed using two methods in parallel for red blood cell (RBC) concentrations of methylated 6-mercaptopurine (6-MP) metabolites. Chemical hydrolysis with high-performance liquid chromatography (HPLC) showed values ranging from 0 to 20,259 pmol/8 x 10(8) RBCs, compared with enzymatic hydrolysis with HPLC that resulted in values ranging from 16 to 22,252 pmol/100 microl packed RBC. Results of the two methods were highly correlated; the coefficient of correlation (r) was equal to 0.93 (95% confidence interval [CI] = 0.87-0.97 [y = 1.12x + 187]). Within series imprecision was 3.1% compared with 6.3%, and between-run imprecision was 10.3% compared with 20.7%, for the enzymatic and chemical methods, respectively. The enzymatic method was found to be more specific and to save time and labor, but with the chemical method methylated metabolites and 6-thioguanine nucleotides (6-TGN), the main active metabolites of azathioprine and 6-MP, can be measured in the same run. The results indicate that methylated 6-MP metabolites mainly exist as ribonucleotides in RBCs.

Citations

Jun 15, 2010·Nucleosides, Nucleotides & Nucleic Acids·S VikingssonC Peterson
Jan 29, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Elise PetitFabrice Morel
Apr 27, 2004·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·B M OliveiraC L Zani
Apr 14, 2009·Therapeutic Drug Monitoring·Svante VikingssonCurt Peterson
Sep 22, 2005·Therapeutic Drug Monitoring·John A Duley, Timothy H J Florin

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