Analysis of micronucleus induction of pyrimethamine in in vitro CHL cells and in in vivo mouse bone marrow cells

Mutagenesis
T Ono, H Yoshimura

Abstract

In vivo and in vitro mutagenicity of pyrimethamine were examined in the micronucleus test. Pyrimethamine strongly induced micronuclei in a dose-dependent manner in the in vitro micronucleus test using the Chinese hamster lung (CHL) cell line, when treated at 0.2-1.6 micrograms/ml for 48 h. The in vivo micronucleus test was carried out in mice after the first, second, third and fourth administration of doses up to 40 mg/kg p.o. The results showed no increased frequency of micronuclei after any treatment, though pyrimethamine was shown to persist at levels > 2 micrograms/ml in plasma after a single oral administration of 50 mg/kg.

Citations

Feb 19, 2000·Teratogenesis, Carcinogenesis, and Mutagenesis·K K Vijayalaxmi, M Vishalakshi
Oct 24, 2002·Teratogenesis, Carcinogenesis, and Mutagenesis·B TuncaR Bilaloglu
Feb 3, 2005·Cell Biology and Toxicology·A Abou-Eisha, M Afifi
Sep 1, 1997·Environmental Toxicology and Pharmacology·T OnoT Ohta
Jun 20, 2006·Mutation Research·Akihiro WakataElisabeth Lorge
Mar 8, 2000·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·C FimognariP Hrelia

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