Analysis of microRNA Expression Profiles Induced by Yiqifumai Injection in Rats with Chronic Heart Failure

Frontiers in Physiology
Yu ZhaoYi Wang

Abstract

Background: Yiqifumai Injection (YQFM) is clinically used to treat various cardiovascular diseases including chronic heart failure (CHF). The efficacy of YQFM for treating heart failure has been suggested, but the mechanism of action for pharmacological effects of YQFM is unclear.Methods:Echocardiography detection, left ventricular intubation evaluation, histopathology and immunohistochemical examination were performed in CHF rats to evaluate the cardioprotective effect of YQFM. Rat miRNA microarray and bioinformatics analysis were employed to investigate the differentially expressed microRNAs.In vitromodels of AngII-induced hypertrophy and t-BHP induced oxidative stress in H9c2 myocardial cells were used to validate the anti-hypertrophy and anti-apoptosis effects of YQFM. Measurement of cell surface area, ATP content and cell viability, Real-time PCR and Western blot were performed.Results:YQFM significantly improved the cardiac function of CHF rats by increasing left ventricular ejection fraction and fractional shortening, decreasing left ventricular internal diameter and enhancing cardiac output. Seven microRNAs which have a reversible regulation by YQFM treatment were found. Among them, miR-21-3p and miR-542-3p are related ...Continue Reading

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Citations

Jan 15, 2021·Heart Failure Reviews·Alieh GholaminejadShaghayegh Haghjooy Javanmard
Jun 29, 2021·Frontiers in Physiology·Jing-Yan HanQiao-Bing Huang

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Datasets Mentioned

BETA
GSE106547

Methods Mentioned

BETA
enzyme linked immunosorbent assay
PCR
PCRs
protein assay
electrophoresis
Assay
chip

Software Mentioned

Pro Plus
DAVID Bioinformatics Resources
Cytoscape
Image
Feature Extraction
String
Genespring
miRwalk
YQFM

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