Analysis of microsatellite alterations in gastric carcinoma using the crypt isolation technique

The Journal of Pathology
Yu-Fei JiaoShin-Ichi Nakamura

Abstract

The crypt isolation technique was used to analyse loss of heterozygosity (LOH) and microsatellite instability (MSI) in gastric carcinomas (36 intestinal type, 17 solid type, and 23 diffuse type) using a polymerase chain reaction assay. Increased LOH frequencies and fractional allelic losses (FAL) were observed in samples prepared using the crypt isolation technique compared with those isolated by the conventional method. A significant increase in LOH was found at several chromosomal loci, and significant differences in FAL were found in patients with intestinal- and solid-type tumours. There was no difference in the frequency of MSI using either technique. In samples prepared by the crypt isolation technique, significant allelic losses (> or =50%) were observed at most loci tested in intestinal- and solid-type tumours, but not in diffuse-type tumours. Significant losses of some of these loci are novel findings for gastric cancer. FAL values were significantly higher in intestinal- and solid-type tumours than in diffuse-type tumours. MSI-high was observed in intestinal- (17%) and solid-type (12%) tumours. The results suggest that the crypt isolation technique is useful for accurate allelic loss analysis in gastric carcinoma and ...Continue Reading

Citations

Jan 1, 2013·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Tomio AraiKaiyo Takubo
Feb 14, 2006·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Yu-Fei JiaoShin-ichi Nakamura
May 30, 2016·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Tamotsu SugaiHiromu Suzuki
Feb 23, 2018·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Tamotsu SugaiHiromu Suzuki
Oct 26, 2016·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Takayuki NagasawaToru Sugiyama
Feb 24, 2010·International Journal of Cancer. Journal International Du Cancer·Tamotsu SugaiKazuyuki Suzuki
Nov 1, 2016·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Takayuki NagasawaToru Sugiyama

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.