Analysis of microsatellites and parentage testing in saltwater crocodiles

The Journal of Heredity
Sally R IsbergChris Moran

Abstract

Fifteen microsatellite loci were evaluated in farmed saltwater crocodiles for use in parentage testing. One marker (C391) could not be amplied. For the remaining 14, the number of alleles per locus ranged from two to 16, and the observed heterozygosities ranged from 0.219 to 0.875. The cumulative exclusion probability for all 14 loci was .9988. the 11 loci that showed the greatest level of polymorphism were used for parentage testing, with an exclusion probability of .9980. With these 11 markers on 107 juveniles from 16 known breeding pairs, a 5.6% pedigree error rate was detected. This level of pedigree error, if consistent, could have an impact on the accuracy of gentic parameter and breeding value estimation. The usefulness of these markers was also evaluated for assigning parentage in situations where maternity, paternity, or both may not be known. In these situations, a 2% error in parentage assignment was predicted. It is therefore recommended that more micro-satellite markers be used in these situations. The use of these microsatellite markers will broaden the scope of a breeding program, allowing progeny to be tested from adults maintained in large breeding lagoons for selection as future breeding animals.

References

Jun 20, 1998·Molecular Ecology·T C MarshallJ M Pemberton
Dec 4, 2002·The Journal of Experimental Zoology·Nancy Nelson FitzsimmonsJacob Gratten
Sep 13, 2003·Molecular Ecology·Adam G Jones, William R Ardren

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Citations

Aug 7, 2012·Immunogenetics·Weerachai JaratlerdsiriJaime Gongora
Sep 9, 2008·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Patricia AmavetCarlos Ignacio Piña
Jun 1, 2019·Journal of Forensic Sciences·M Katherine Moore, Kim Frazier
Apr 2, 2014·The Journal of Heredity·Deyla P OliveiraTomas Hrbek

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