PMID: 9426591Jan 14, 1998Paper

Analysis of microspore-specific promoters in transgenic tobacco

Plant Molecular Biology
J B CustersM M van Lookeren Campagne

Abstract

In order to modify the early stages of pollen development in a transgenic context microspore-specific promoters are required. We tested two putatively microspore-specific promoters, the Bp4 promoter from rapeseed and the NTM19 promoter from tobacco. Expression of the gus and barnase reporter genes under the control of these two promoters was studied in transgenic tobacco. Contrary to expectations, the Bp4 promoter became active only after the first pollen mitosis, and not in the microspores. The NTM19 promoter turned out to be highly microspore-specific and directed very high levels of gus expression to the unicellular microspores. The NTM19-barnase transgene caused cell-autonomous death at the mid-unicellular microspore stage, whereas Bp4-barnase induced cell ablation of early to mid-bicellular pollen. Both promoter-barnase transgenes did not affect the sporophyte and were inherited through the female germline. These results show that both the NTM19 and Bp4 promoters are expressed only in the male germline, and that the NTM19 promoter is an excellent tool to direct high levels of transgene expression exclusively to the microspores. This may have important biotechnological applications.

Citations

May 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Weichang YuJames A Birchler
May 28, 2008·Cytogenetic and Genome Research·J A BirchlerF Han
May 14, 2017·The Plant Journal : for Cell and Molecular Biology·Xiaohong YanWenhui Wei
Dec 6, 2011·Annual Review of Plant Biology·Robert T GaetaJames A Birchler
Feb 11, 2005·The Plant Journal : for Cell and Molecular Biology·Alexander A T JohnsonEmmanuel Guiderdoni
Jun 19, 2008·PloS One·Evelina EdelweissSergey M Deyev
Oct 28, 2015·Plant Biotechnology Journal·Reginald J MillwoodCharles Neal Stewart
Jan 22, 2015·Development·Colette A ten HoveDolf Weijers

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