Analysis of MinD mutations reveals residues required for MinE stimulation of the MinD ATPase and residues required for MinC interaction

Journal of Bacteriology
Huaijin ZhouJ Lutkenhaus

Abstract

The MinD ATPase is critical to the oscillation of the Min proteins, which limits formation of the Z ring to midcell. In the presence of ATP, MinD binds to the membrane and recruits MinC, forming a complex that can destabilize the cytokinetic Z ring. MinE, which is also recruited to the membrane by MinD, displaces MinC and stimulates the MinD ATPase, resulting in the oscillation of the Min proteins. In this study we have investigated the role of lysine 11, present in the deviant Walker A motif of MinD, and the three residues in helix 7 (E146, S148, and D152) that interact electrostatically with lysine 11. Lysine 11 is required for interaction of MinD with the membrane, MinC, MinE, and itself. In contrast, the three residues in helix 7 that interact with lysine 11 are not required for binding to the membrane or activation of MinC. They are also not required for MinE binding; however, they are required for MinE to stimulate the MinD ATPase. Interestingly, the D152A mutant self-interacts, binds to the membrane, and recruits MinC and MinE in the presence of ADP as well as ATP. This mutant provides evidence that dimerization of MinD is sufficient for MinD to bind the membrane and recruit its partners.

References

Feb 1, 1993·Journal of Bacteriology·E Bi, J Lutkenhaus
Jan 12, 1999·Journal of Bacteriology·J C ChenJon Beckwith
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·David M Raskin, P A de Boer
Oct 9, 1999·Journal of Bacteriology·David M Raskin, P A de Boer
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·Z HuJ Lutkenhaus
Feb 26, 2000·Annual Review of Genetics·Lawrence I RothfieldJ García-Lara
Feb 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·X FuLawrence I Rothfield
Mar 15, 2001·FEBS Letters·S C Cordell, Jan Löwe
Dec 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·H Meinhardt, P A de Boer
Jan 25, 2002·Biophysical Journal·Karsten Kruse
Mar 28, 2002·Journal of Molecular Biology·Detlef D LeipeL Aravind
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Zonglin HuJ Lutkenhaus
Nov 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tim H SzetoGlenn F King
Dec 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Kyoko SuefujiDebabrata RayChaudhuri
Apr 5, 2003·Molecular Microbiology·J Lutkenhaus, M Sundaramoorthy
Apr 5, 2003·The Journal of Biological Chemistry·Eugenia MileykovskayaWilliam Dowhan
May 27, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yu-Ling ShihLawrence I Rothfield
Jul 29, 2003·The Journal of Biological Chemistry·Tim H SzetoGlenn F King
Oct 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Kerwyn Casey HuangNed S Wingreen
Feb 20, 2004·Journal of Bacteriology·Huaijin Zhou, J Lutkenhaus

Citations

Jul 5, 2013·Nature Structural & Molecular Biology·Gert Bange, Irmgard Sinning
Nov 13, 2008·The Journal of Biological Chemistry·Jeffrey M BoydDiana M Downs
Nov 10, 2007·Molecular Plant-microbe Interactions : MPMI·Yongsheng JinTao Wang
Jun 8, 2006·Annual Review of Biochemistry·Katharine A Michie, Jan Löwe
Jan 8, 2016·Proceedings of the National Academy of Sciences of the United States of America·Dominik ThalmeierErwin Frey
Aug 14, 2015·Molecular Microbiology·Kyung-Tae ParkJ Lutkenhaus
Jun 8, 2012·Trends in Microbiology·J Lutkenhaus
Mar 9, 2005·Molecular Microbiology·Sebastien Pichoff, J Lutkenhaus
Jun 2, 2012·Molecular Microbiology·Kyung-Tae ParkJ Lutkenhaus
Sep 12, 2013·Molecular Systems Biology·Barbara Di VenturaVictor Sourjik
Dec 23, 2009·Molecular Microbiology·Cheng-Wei HsiehYu-Ling Shih
Apr 12, 2013·Environmental Microbiology·Yu-Ling Shih, Min Zheng
Jul 12, 2005·Current Biology : CB·Nathan W Goehring, Jon Beckwith
May 8, 2007·Biophysical Journal·Eric N Cytrynbaum, Brandon D L Marshall
Feb 8, 2018·The Journal of Biological Chemistry·Kyung-Tae ParkJ Lutkenhaus
Oct 6, 2018·Bioscience, Biotechnology, and Biochemistry·Lei KeJian-Chun Guo
Apr 5, 2007·Physical Biology·Elisabeth Fischer-FriedrichKarsten Kruse
Mar 28, 2006·The Journal of Biological Chemistry·Didier SoulatChristophe Grangeasse
Nov 29, 2016·Molecular Microbiology·Joshua S MacCreadyDaniel C Ducat
May 28, 2020·Open Biology·Navaneethan PalanisamyBarbara Di Ventura
Jul 22, 2015·The Journal of Biological Chemistry·Eric J CamireDeborah L Perlstein
Feb 28, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Miguel Á Pérez-RodríguezXianwu Guo
Jul 19, 2019·Cellular and Molecular Life Sciences : CMLS·Beatrice RammPetra Schwille

Related Concepts

MinD protein, E coli
MinE protein, E coli
MinC protein, E coli
Magnesium ADP
DNA-dependent ATPase
Adenosine Triphosphate, Chromium Ammonium Salt
DNA Mutational Analysis
Alkalescens-Dispar Group
Cell Surface Proteins
Plasma Protein Binding Capacity

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