Abstract
To clarify distinct genetic profiles of colorectal cancers based on tumor location (left- and right-sided), we evaluated the status of loss of heterozygosity (LOH), CpG islands methylation phenotype (CIMP), microsatellite instability (MSI), and mutations of p53, Ki-ras, and APC genes in 119 colorectal cancers. Statuses of LOH (at 5q, 8p, 17p, 18q, and 22q), MSI, and CIMP (MINT1, MINT2, MINT31, MLH-1, MGMT, p14, p16, and RASSF1A) were determined using microsatellite polymerase chain reaction and methylation-specific polymerase chain reaction coupled with a crypt isolation method, respectively. In addition, mutations of p53, Ki-ras, and APC genes were also examined. LOH, MSI, and CIMP status allowed us to classify samples into two groups: low or negative and high or positive. Whereas the frequency of p53 mutations in the LOH-high status was significantly higher in left-sided cancers than in right-sided cancers, CIMP-high in the LOH-high status and MSI-positive status were more frequently found in right-sided cancers compared with left-sided cancers. Finally, location-specific methylated loci were seen in colorectal cancers: type I (dominant in right-sided cancer) and type II (common in both segments of cancer). Our data confirm t...Continue Reading
References
Jun 1, 1990·Cell·E R Fearon, B Vogelstein
Sep 1, 1988·The New England Journal of Medicine·B VogelsteinJ L Bos
Sep 1, 1967·Annals of Surgery·R B TurnbullJ Spratt
Jan 1, 1994·Gastroenterology·S NakamuraI Kino
Jan 1, 1993·Gastroenterology·S M BellP Quirke
Apr 10, 1997·Nature·C LengauerB Vogelstein
Jan 8, 1998·International Journal of Cancer. Journal International Du Cancer·J BreivikG Gaudernack
Jan 1, 1999·Nature·C LengauerB Vogelstein
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·M ToyotaJ P Issa
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·M ToyotaJ P Issa
Mar 8, 2000·Archives of Pathology & Laboratory Medicine·T SugaiC Itoh
Mar 24, 2000·Pathology, Research and Practice·T SugaiR Katoh
Oct 29, 2000·The American Journal of Gastroenterology·W A BleekerJ T Plukker
Nov 4, 2000·International Journal of Cancer. Journal International Du Cancer·T SugaiC Itoh
Jan 10, 2001·Current Opinion in Oncology·A Lindblom
Apr 6, 2001·Diseases of the Colon and Rectum·W A BleekerJ T Plukker
Sep 11, 2001·The American Journal of Pathology·A RashidS R Hamilton
Oct 11, 2001·Oncogene·T SugaiT Higuchi
Dec 1, 2001·Gastroenterology·Y MiyakuraH Nagai
May 2, 2002·Gastroenterology·Nicholas HawkinsRobyn Ward
May 7, 2002·International Journal of Cancer. Journal International Du Cancer·Wade S SamowitzMartha L Slattery
May 10, 2002·The American Journal of Pathology·Annie On-On ChanAsif Rashid
Jul 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Gillian SmithC Roland Wolf
Aug 29, 2002·Gastroenterology·Jeremy R JassBarbara A Leggett
Sep 7, 2002·International Journal of Cancer. Journal International Du Cancer·Barry Iacopetta
Nov 2, 2002·The Journal of Molecular Diagnostics : JMD·Tamotsu SugaiMotohiro Ishii
May 20, 2003·The Journal of Pathology·Tamostu SugaiKazuyuki Suzuki
Feb 21, 2004·Carcinogenesis·Margreet LüchtenborgAnton F P M de Goeij
Jul 13, 2004·Gut·T KambaraB A Leggett
Dec 4, 2004·International Journal of Cancer. Journal International Du Cancer·Tamotsu SugaiShin-Ichi Nakamura
Citations
Oct 12, 2012·Surgical Endoscopy·Steven K NakaoD Mark Zebley
Aug 13, 2013·Current Gastroenterology Reports·Michael Tadros, Joseph C Anderson
Apr 16, 2013·Nature Medicine·Felipe De Sousa E MeloLouis Vermeulen
Jan 1, 2010·Journal of the National Cancer Institute·Hermann BrennerUlrike Haug
Jan 1, 2010·Journal of the National Cancer Institute·Nancy N Baxter, Linda Rabeneck
Feb 1, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Joo Mi YiNita Ahuja
Jun 7, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Christopher G SmithJeremy P Cheadle
Oct 16, 2007·BMC Cancer·Anders LorentzenCathy Mitchelmore
Jan 6, 2010·Annals of Surgical Oncology·Young-Ho KimJae J Kim
Nov 13, 2008·PloS One·Katsuhiko NoshoShuji Ogino
Oct 11, 2012·PloS One·Kinga TóthBéla Molnár
Aug 10, 2012·American Journal of Public Health·Amanda I PhippsPolly A Newcomb
Aug 19, 2007·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Francesco Di FabioMark Trifiro
May 21, 2014·Digestive Diseases and Sciences·Amy L CisykKirk J McManus
Nov 17, 2009·Gastroenterology·Hermann BrennerMichael Hoffmeister
Dec 15, 2015·Expert Review of Molecular Diagnostics·Kinga TóthBéla Molnár
Feb 5, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Petros Christakis Papagiorgis
May 7, 2014·BioMed Research International·Inés OmraneAmel Benammar-Elgaaied
Apr 22, 2008·Cancer Science·Jin C KimYong S Kim
Sep 18, 2008·International Journal of Cancer. Journal International Du Cancer·Yong Ho KimYoung S Kim
Jun 27, 2015·Chinese Journal of Cancer·Feng WangRui-Hua Xu
May 1, 2015·International Journal of Colorectal Disease·Takahiro HorimatsuHisao Tajiri
May 20, 2008·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Guoren DengYoung S Kim
Mar 20, 2014·Cancer Biology & Therapy·Joanna H M TongKa Fai To
Feb 24, 2015·Frontiers in Oncology·Francesco CaiazzaKieran Sheahan
Dec 10, 2014·Cancer Causes & Control : CCC·Xu Yao, Zhong Tian
Oct 6, 2012·Nutrition and Cancer·Helen F ClapinJane S Heyworth
May 10, 2016·Oncogene·E FesslerL Vermeulen
Apr 16, 2015·PloS One·Younghoon KimGyeong Hoon Kang
Oct 6, 2016·Journal of Gastroenterology·Tamotsu SugaiTakayuki Matsumoto
Dec 7, 2016·Nature Reviews. Clinical Oncology·Cornelis J A PuntLouis Vermeulen
Mar 23, 2017·Clinical Endoscopy·Yu Mi Lee, Kyu Chan Huh
Feb 23, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Mayank JauhriShyam Aggarwal
Feb 21, 2009·Inflammatory Bowel Diseases·M M H ClaessenF P Vleggaar
Aug 20, 2011·Journal of Medical Screening·Thomas J W LeeColin J Rees
Jul 29, 2015·Cancer·Shaham BegKhawla S Al-Kuraya
Jan 6, 2007·Histopathology·J R Jass
May 24, 2007·British Journal of Cancer·F MansillaT F Orntoft
Aug 19, 2007·Journal of Clinical Gastroenterology·Elizabeth T JacobsMaría Elena Martínez
Feb 24, 2010·International Journal of Cancer. Journal International Du Cancer·Tamotsu SugaiKazuyuki Suzuki
Jun 15, 2010·Genes, Chromosomes & Cancer·Cristina AlbuquerqueRiccardo Fodde
Aug 1, 2013·British Journal of Cancer·J M BaeG H Kang
Apr 21, 2011·Expert Review of Anticancer Therapy·Richard M HoffmanRobert L Rhyne
Feb 9, 2012·The American Journal of Surgical Pathology·Reetesh K PaiRish K Pai
Dec 17, 2009·Diseases of the Colon and Rectum·Frank BenedixUNKNOWN Colon/Rectum Carcinomas (Primary Tumor) Study Group
Jan 10, 2012·Diseases of the Colon and Rectum·Jeong M BaeGyeong H Kang
Dec 19, 2019·Journal of the National Cancer Institute·Paul R BarberTony Ng
Apr 19, 2017·Oncotarget·Kunrong ChengJean-Pierre Raufman
Mar 5, 2016·Asia-Pacific Journal of Clinical Oncology·Hsueh-Ju LuHao-Wei Teng
May 27, 2020·Cancer Medicine·Matilda HolmCaj Haglund