Analysis of molecular markers as IL-12, IL-22 and IFN-γ in correlation with a clinical course in patients with psoriasis.

International Journal of Occupational Medicine and Environmental Health
Magdalena KutwinAnna Woźniacka

Abstract

As a chronic, recurrent, immunologically mediated systemic disease and a common cause of dermatological problems, psoriasis is often a subject of scientific research. Skin changes located on the hands can cause difficulties and limitations in the performance of professional activities, especially manual ones. The main role in pathogenesis is played by immunological factors - improper functioning of the components of the immune system, among others, T lymphocytes and cytokines like interleukin-12 (IL-12), interleukin-22 (IL-22) and interferon gamma (IFN-γ). The obtained tissue and blood were destined for RNA isolation. The RNA was then subjected to a reverse transcription reaction. The relative gene expression level was evaluated by the real-time polymerase chain reaction for <i>IL-12B</i>, <i>IL-22</i> and <i>IFN-γ</i> genes, and presented as the relative quantification (RQ) value, relative to the reference gene <i>GAPDH</i>. In addition, a correlation analysis of the expression level of selected genes with the clinical course of the disease, as assessed by the <i>Psoriasis Area and Severity Index</i> (PASI), the<i> Body Surface Area</i> (BSA) and the <i>Dermatology Life Quality Index</i> (DLQI) scores was performed. Statistica...Continue Reading

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