PMID: 15225135Jul 1, 2004Paper

Analysis of morphological and functional maturation of neoislets generated in vitro from pancreatic ductal cells and their suitability for islet banking and transplantation

The Journal of Endocrinology
M R KatdareP B Parab

Abstract

The pancreatic ductal stem cells are known to differentiate into islets of Langerhans; however, their yield is limited and the islet population is not defined. Therefore, the aims of the present study were to improvise a methodology for obtaining large numbers of islets in vitro and to characterize their morphological and functional status for islet cell banking and transplantation. Pancreatic ductal epithelial cell cultures were set in serum-free medium. Monolayers of epithelial cells in culture gave rise to islet-like clusters within 3-4 weeks. The identity of neoislets was confirmed by dithizone staining and analysis of the gene expression for endocrine markers by reverse transcriptase-polymerase chain reaction (RT-PCR). The islet population obtained was analysed by image analysis and insulin secretion in response to secretagogues. The cellular extracts from neoislets were immunoreactive to anti-insulin antibody and expressed insulin, glucagon, GLUT-2, PDX-1 and Reg-1 genes. The islets generated within 3-4 weeks exhibited a mixed population of large- and small-sized islets with clear cut dichotomy in the pattern of their insulin secretion in response to L-arginine and glucose. These neoislets maintained their structural and ...Continue Reading

Citations

Apr 9, 2005·Cell Death and Differentiation·A-M JamalL Rosenberg
Jun 10, 2010·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Marjorie FanjulEtienne Hollande
May 12, 2007·The Review of Diabetic Studies : RDS·Meghana Kanitkar, Ramesh Bhonde
Dec 23, 2011·The Review of Diabetic Studies : RDS·Savita P Datar, Ramesh R Bhonde
Apr 5, 2012·Evidence-based Complementary and Alternative Medicine : ECAM· AnsarullahA V Ramachandran
Sep 22, 2011·Islets·Vijayalakshmi VenkatesanRamesh R Bhonde
Apr 6, 2011·Experimental Biology and Medicine·Sashi G KiranVijayalakshmi Venkatesan
May 11, 2005·Cell Transplantation·Meenal Banerjee, Ramesh Bhonde
Nov 9, 2014·European Journal of Orthodontics·Anna-Liisa Svedström-OristoPentti Alanen
Oct 4, 2011·Colloids and Surfaces. B, Biointerfaces·Evan A DubielPatrick Vermette

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