Analysis of Mosquito-Borne Flavivirus Superinfection in Culex tritaeniorhynchus (Diptera: Culicidae) Cells Persistently Infected with Culex Flavivirus (Flaviviridae)

Journal of Medical Entomology
Ryusei KuwataKyoko Sawabe

Abstract

Superinfection exclusion is generally defined as a phenomenon in which a pre-existing viral infection prevents a secondary viral infection; this has also been observed in infections with mosquito-borne viruses. In this study, we examined the superinfection exclusion of the vertebrate-infecting flaviviruses, Japanese encephalitis virus (JEV) and dengue virus (DENV), by stable and persistent infection with an insect-specific flavivirus, Culex flavivirus (CxFV), in a Culex tritaeniorhynchus Giles cell line (CTR cells). Our experimental system was designed based on the premise that wild Cx. tritaeniorhynchus mosquitoes naturally infected with CxFV are superinfected with JEV by feeding on JEV-infected animals. As a result, we found no evidence of the superinfection exclusion of both JEV and DENV by pre-existing CxFV infection at the cellular level. However, JEV superinfection induced severe cytopathic effects on persistently CxFV-infected CTR cells. These observations imply the possibility that JEV superinfection in CxFV-infected Cx. tritaeniorhynchus mosquitoes has an adverse effect on their fitness.

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Citations

Sep 18, 2015·Viruses·Bethany G BollingNikos Vasilakis
Oct 30, 2015·Journal of Medical Entomology·Christina M NewmanTony L Goldberg
Feb 9, 2017·Memórias do Instituto Oswaldo Cruz·Miguel A SaldañaGrant L Hughes
Dec 16, 2017·Tropical Animal Health and Production·Kiven KumarAhmad Khusaini Mohd Kharip Shah
Mar 10, 2018·Viruses·William H PalmerRonald P van Rij
Mar 21, 2018·Microbiome·Morgane GuéganClaire Valiente Moro
Sep 20, 2019·Viruses·Eric AgboliEsther Schnettler
Aug 31, 2019·Emerging Microbes & Infections·Vincent HouéAnna-Bella Failloux
Aug 1, 2020·Microbiology Resource Announcements·Marielton Dos Passos CunhaPaolo Marinho de Andrade Zanotto
Jan 1, 2018·Biologie aujourd'hui·Katy Heu, Mathilde Gendrin
Jan 9, 2019·Frontiers in Microbiology·Isabella Dias da SilveiraRafael Maciel-de-Freitas
Jan 12, 2019·Virus Genes·Pontus ÖhlundAnne-Lie Blomström
Jun 11, 2020·Memórias do Instituto Oswaldo Cruz·Silvina GoenagaSilvana Del Carmen Levis
Sep 26, 2019·Frontiers in Microbiology·Francesca ScolariMariangela Bonizzoni
Aug 14, 2020·Viruses·Geovani de Oliveira RibeiroÉlcio Leal
May 7, 2021·Journal of Virology·Edward I PattersonNaomi L Forrester
Sep 17, 2021·Virology Journal·Tomo DaidojiTakaaki Nakaya

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