Analysis of multispectral imaging with the AstroPath platform informs efficacy of PD-1 blockade.

Science
Sneha BerryJanis M Taube

Abstract

Next-generation tissue-based biomarkers for immunotherapy will likely include the simultaneous analysis of multiple cell types and their spatial interactions, as well as distinct expression patterns of immunoregulatory molecules. Here, we introduce a comprehensive platform for multispectral imaging and mapping of multiple parameters in tumor tissue sections with high-fidelity single-cell resolution. Image analysis and data handling components were drawn from the field of astronomy. Using this "AstroPath" whole-slide platform and only six markers, we identified key features in pretreatment melanoma specimens that predicted response to anti-programmed cell death-1 (PD-1)-based therapy, including CD163+PD-L1- myeloid cells and CD8+FoxP3+PD-1low/mid T cells. These features were combined to stratify long-term survival after anti-PD-1 blockade. This signature was validated in an independent cohort of patients with melanoma from a different institution.

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Citations

Aug 11, 2021·Cancer Cell·Ming Y LuFaisal Mahmood
Oct 6, 2021·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Lynette M Sholl
Aug 27, 2021·Cancer Immunology Research·Nicolas A GiraldoJanis M Taube

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