Analysis of mutagens from cooked foods by directly combined liquid chromatography-mass spectrometry

Environmental Health Perspectives
C G EdmondsJ A McCloskey

Abstract

Directly combined high performance liquid chromatography-mass spectrometry (LC/MS) has been studied as a method of analysis of heterocyclic aromatic mutagens in cooked foods, in the parts per billion concentration range. Identification and semiquantitative estimation of mutagens is based on accurate measurement of chromatographic retention (k') and molecular weight-selective detection of mutagens, which are protonated during passage of the chromatographic eluant into a thermospray interface of a quadrupole mass spectrometer. Standard chromatographic retention (k') values in two reversed-phase systems and data from thermospray mass spectra from nine mutagens are reported. An isolation scheme employing CH3OH extraction, acid-base partition, cellulose-trisulfo-Cu-phthalocyanine adsorption, and normal-phase HPLC was used prior to LC/MS analysis. Initial applications have been demonstrated in the analysis of 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) and 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ) in broiled salmon flesh. Levels measured were estimated to be in the range 0.2 to 0.4 microgram/kg IQ and 0.4 to 0.9 microgram/kg MeIQ. The method is judged to be generally applicable with minimal sample prefractionation to detect...Continue Reading

References

Aug 1, 1986·Environmental Health Perspectives·S Nishimura
Jan 1, 1984·Carcinogenesis·J S FeltonF T Hatch

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Citations

Aug 1, 1986·Environmental Health Perspectives·S Nishimura
Mar 22, 2001·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·G A Keating, K T Bogen
Oct 1, 1994·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·B Stavric
Sep 1, 1993·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·K Skog
Jun 1, 1988·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·R J TureskyH Milon
Aug 31, 2002·Journal of Mass Spectrometry : JMS·Francisca ToribioMaria Teresa Galceran
Mar 1, 2016·Comprehensive Reviews in Food Science and Food Safety·Monika Gibis
Dec 5, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·P Pais, M G Knize
Dec 5, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·F ToribioL Puignou

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