Analysis of mutation at the hprt locus in human T lymphocytes

Toxicology Letters
B LambertS M Hou

Abstract

Studies of mutation at the hypoxanthine phosphoribosyl transferase (hrpt) locus in human T-cells have the potential to elucidate the molecular basis of in vivo mutagenesis, reveal exposure dependent changes in ther background frequency of mutation, and provide knowledge on individual sensitivity. Styrene exposed lamination workers in Bohemia showed a significantly higher frequency of hprt mutant cells than Swedish control populations studied simultaneously. In a study of 47 healthy, non-smoking male bus maintenance workers exposed to diesel exhausts, soot and oil, and 22 unexposed controls, a significant correlation (P = 0.008) was obtained between the levels of aromatic DNA adducts and frequencies of hprt-mutant T-cells. In the group of workers with the highest exposure, subjects with glutathione S-transferase (GSTM1) deficiency showed significantly higher (P < 0.05) frequency of hprt mutant T-cells than GSTM1-positive subjects. The highest adduct levels were found in subjects with the combined genotype of GSTM1 and NAT2 deficiency (GSTM1-negative slow acetylators). These results indicate that GSTM1 and NAT2 genotypes may play a role in determining the individual levels of hprt mutation and DNA adducts. Using PCR-based screeni...Continue Reading

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Citations

Apr 10, 2002·Environmental Health Perspectives·Marià PitarqueRicard Marcos
Apr 18, 2008·Environmental and Molecular Mutagenesis·Irene M JonesDavid O Nelson
Jan 21, 2006·Pharmacogenetics and Genomics·Lucia MiglioreAri Hirvonen
Dec 1, 1995·Journal of Medical Genetics·P GuldbergA de la Chapelle
Jul 10, 2019·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·M I BantonS S Sarang

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