Analysis of mutually exclusive alternatively spliced serpin-1 isoforms and identification of serpin-1 proteinase complexes in Manduca sexta hemolymph.

The Journal of Biological Chemistry
Emily J RaganM R Kanost

Abstract

Mutually exclusive alternative splicing produces transcripts for 12 serpin-1 isoforms in Manduca sexta that differ only in the region encoding the carboxyl-terminal 36-40-amino acid residues. This variable region includes the reactive center loop, which determines the inhibitory selectivity of the serpin. We investigated mRNA levels of individual serpin-1 isoforms by quantitative PCR. The 12 isoforms were expressed at similar levels in hemocytes, but in fat body isoform B mRNA was present at significantly higher levels than isoforms C, D, E, F, G, J, K, and Z. To investigate the presence of individual serpin-1 isoforms in plasma we used immunoaffinity purification of serpin-1 isoforms from M. sexta plasma, followed by two-dimensional PAGE and identification of protein spots by digestion with a series of proteinases and analysis of the resulting peptides by MALDI-TOF/TOF. We identified nine of the 12 serpin-1 isoforms and, through analysis of putative serpin-1-proteinase complexes, identified three endogenous M. sexta proteinase targets of serpin-1. Our results suggest that M. sexta serpin-1 isoforms A, E, and J can inhibit hemolymph proteinase 8, which activates the cytokine spätzle. At least one isoform of serpin-1 can inhibit...Continue Reading

References

Dec 5, 1991·European Journal of Biochemistry·T Sasaki
Jul 1, 1995·Insect Biochemistry and Molecular Biology·A M PetersonM A Wells
Feb 1, 1995·Insect Biochemistry and Molecular Biology·M R KanostE Willott
Jun 16, 1999·Structure·J LiE J Goldsmith
Nov 1, 2002·Developmental Cell·Ennio De GregorioPaul T Brey
Nov 29, 2002·The EMBO Journal·Petros LigoxygakisJean-Marc Reichhart
Dec 5, 2003·Current Biology : CB·Petros LigoxygakisJean-Marc Reichhart
Dec 12, 2003·Developmental Cell·Carl HashimotoDonald Morisato
Mar 18, 2004·Archives of Insect Biochemistry and Physiology·K S BrandtN Wisnewski
Jun 10, 2005·Insect Biochemistry and Molecular Biology·Haobo JiangMichael R Kanost
Jul 25, 2006·The Journal of Biological Chemistry·Huaping TangCarl Hashimoto
Oct 27, 2006·Proceedings of the National Academy of Sciences of the United States of America·Kristin MichelFotis C Kafatos
Nov 3, 2006·Current Opinion in Structural Biology·James C Whisstock, Stephen P Bottomley
Aug 1, 2007·Journal of Thrombosis and Haemostasis : JTH·J C RauF C Church
Aug 31, 2007·Genome Biology·Zhen ZouHaobo Jiang
Jan 16, 2008·The Journal of Biological Chemistry·Chan-Hee KimBok Luel Lee
Jul 17, 2008·The Journal of Biological Chemistry·Hongnan KanBok Luel Lee
Sep 20, 2008·Developmental Biology·Christoph ScherferBruno Lemaitre
Oct 16, 2008·Developmental Cell·Huaping TangCarl Hashimoto
Jan 20, 2009·Genomics·Zhen ZouHaobo Jiang

❮ Previous
Next ❯

Citations

Apr 10, 2013·Insect Biochemistry and Molecular Biology·Chansak SuwanchaichindaMichael R Kanost
Dec 31, 2011·Thrombosis Research·Akio Saito
Oct 16, 2012·Insect Biochemistry and Molecular Biology·Jayne M ChristenMichael R Kanost
Sep 8, 2016·Seminars in Cell & Developmental Biology·David A MeekinsKristin Michel
Mar 12, 2016·International Journal of Insect Science·Ming-Hui JinChao-Bin Xue

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.