PMID: 11911090Mar 26, 2002Paper

Analysis of N-linked oligosaccharides: progress towards the characterisation of glycoprotein-linked carbohydrates

Biomolecular Engineering
J CharlwoodP Camilleri

Abstract

The covalent attachment of carbohydrate to proteins is a very common co- or post-translational event in the biosynthesis of glycoproteins. The type and heterogeneity of these oligosaccharides can affect a range of physico-chemical and biological properties of a glycoprotein. Thus the development of sensitive, reliable and robust analytical methods for carbohydrate analysis is important in the pharmaceutical industry, especially in the recombinant production of experimental and therapeutic glycoproteins. In this report we have reviewed methodology for the in-gel enzymatic release of N-linked oligosaccharides from glycoproteins separated by electrophoresis. These oligosaccharides are derivatised by reductive amination using 3-acetamido-6-aminoacridine (AA-Ac), a novel, highly fluorescent probe. A major advantage of this technique is that glycan derivatives are amenable to analysis by an array of chromatographic and mass spectrometric methods, allowing the resolution and characterisation of a wide variety of glycan structures. It is hoped that in due course the methodology described will be applied to proteomics studies, especially in identifying the role of carbohydrate in protein function and disease.

References

Jan 1, 1985·Annual Review of Biochemistry·R Kornfeld, S Kornfeld
Jul 28, 1995·Science·R D FleischmannJ M Merrick
Oct 25, 1996·Science·A GoffeauS G Oliver
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·A ShevchenkoM Mann
Sep 8, 1997·Archives of Microbiology·S Moens, J Vanderleyden
Jun 25, 1998·Electrophoresis·N H PackerA A Gooley
Jan 14, 2000·Nature Biotechnology·M G Pluskal
Apr 14, 2000·Analytical Chemistry·J CharlwoodP Camilleri
Apr 14, 2000·Analytical Chemistry·J CharlwoodP Camilleri

❮ Previous
Next ❯

Citations

Feb 3, 2004·Food Additives and Contaminants·S J LahtinenJ T Ahokas
Jan 28, 2009·Acta Crystallographica. Section D, Biological Crystallography·Thomas Lütteke
Jul 25, 2006·Journal of Molecular Biology·Sandra GoettschCristina Hartmann-Fatu
Apr 16, 2004·Molecular & Cellular Proteomics : MCP·Troels Zakarias KristiansenAkhilesh Pandey
Dec 31, 2010·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Vera IgnjatovicPaul Monagle
May 24, 2006·Electrophoresis·Michael J LittlePieter K Roos
Feb 11, 2005·Yeast·Sue Macauley-PatrickLinda M Harvey

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.