Analysis of Neat Biofluids Obtained During Cardiac Surgery Using Nanoparticle Tracking Analysis: Methodological Considerations

Frontiers in Cell and Developmental Biology
Andrew I U ShearnGiovanni Biglino

Abstract

Small extracellular vesicles (sEVs) are those nanovesicles 30-150 nm in size with a role in cell signalling and potential as biomarkers of disease. Nanoparticle tracking analysis (NTA) techniques are commonly used to measure sEV concentration in biofluids. However, this quantification technique can be susceptible to sample handing and machine settings. Moreover, some classes of lipoproteins are of similar sizes and could therefore confound sEV quantification, particularly in blood-derived preparations, such serum and plasma. Here we have provided methodological information on NTA measurements and systematically investigated potential factors that could interfere with the reliability and repeatability of results obtained when looking at neat biofluids (i.e., human serum and pericardial fluid) obtained from patients undergoing cardiac surgery and from healthy controls. Data suggest that variables that can affect vesicle quantification include the level of contamination from lipoproteins, number of sample freeze/thaw cycles, sample filtration, using saline-based diluents, video length and keeping the number of particles per frame within defined limits. Those parameters that are of less concern include focus, the "Maximum Jump" set...Continue Reading

References

Aug 3, 2002·Nature Reviews. Immunology·Clotilde ThérySebastian Amigorena
May 24, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Rebecca A DragovicIan L Sargent
Sep 7, 2013·Journal of Extracellular Vesicles·Chris GardinerIan L Sargent
Sep 7, 2013·Journal of Extracellular Vesicles·Kenneth W WitwerFred Hochberg
Jun 22, 2014·Journal of Immunological Methods·Laurent MullerTheresa L Whiteside
Jan 4, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sybren L N MaasEsther N M Nolte-'t Hoen
Apr 15, 2015·Vascular Pharmacology·Costanza EmanueliSusmita Sahoo
Apr 18, 2015·Journal of the American College of Cardiology·Jose M VicencioSean M Davidson
Jul 22, 2015·Journal of Extracellular Vesicles·Richard J LobbAndreas Möller
Jan 3, 2016·Clinica Chimica Acta; International Journal of Clinical Chemistry·Estibaliz AlegreAlvaro González
Oct 28, 2016·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Francesco CappelloStefano Fais
Nov 9, 2016·Scientific Reports·Steffi BoschJean-Marie Bach
Feb 6, 2017·Molecular Therapy : the Journal of the American Society of Gene Therapy·Cristina BeltramiCostanza Emanueli
Feb 19, 2017·Blood·Salomon ManierIrene M Ghobrial
Apr 16, 2017·Annals of Neurology·Igor SelmajMarcin P Mycko
May 13, 2017·Circulation Research·Frank A W CoumansRienk Nieuwland
Aug 15, 2017·Journal of Extracellular Vesicles·Beate VestadReidun Øvstebø
Feb 15, 2018·Cellular and Molecular Life Sciences : CMLS·Nasibeh KarimiCecilia Lässer
Jun 29, 2018·Frontiers in Oncology·Matteo GiuliettiRodolfo Montironi

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Citations

Jan 26, 2021·Molecular Biotechnology·Talitha Keren KurianNirmal Mazumder
Mar 22, 2021·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sezin AdayCostanza Emanueli

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Methods Mentioned

BETA
coronary artery bypass
size exclusion chromatography
ELISA

Software Mentioned

NTA

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