Analysis of neomycin using an improved liquid chromatographic method combined with pulsed electrochemical detection

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
N H ZawillaErwin Adams

Abstract

An isocratic liquid chromatographic method with pulsed electrochemical detection is described for the determination of neomycin in the presence of its impurities. The mobile phase is composed of an aqueous solution containing 35 g/l of sodium sulphate, 1 g/l of sodium 1-octanesulfonate, 14 ml/l of tetrahydrofuran (THF) and 50 ml/l of 0.2 M phosphate buffer pH 3.0. Sodium hydroxide was added post column to enhance the detection. An investigation of different reversed-phase columns indicated that the Discovery (C18 5 microm, 250 mm x 4.6 mm I.D.) column was the most suitable. The proposed method shows high efficiency, allowing the separation of the main component neomycin B from neomycin C and 15 other impurities. A central composite design was used to assess the robustness of the method. The method showed good selectivity, repeatability, linearity and sensitivity. This method was applied to analyse commercial samples.

Citations

Nov 6, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xin LuShulin Zhao
Feb 17, 2009·Biosensors & Bioelectronics·Noemí de-los-Santos-AlvarezPaulino Tuñón-Blanco
May 8, 2013·Macromolecular Rapid Communications·Guodong ZhouXiaoqiang Chen
Mar 18, 2009·Medicinal Research Reviews·Shan JinBinghe Wang
Feb 10, 2012·Biosensors & Bioelectronics·Xiaoling WuChuanlai Xu
Apr 14, 2009·Journal of Pharmaceutical and Biomedical Analysis·A L HuidobroC Barbas
Jul 23, 2013·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Vladimir V ApyariYury A Zolotov
Mar 8, 2020·Journal of the Science of Food and Agriculture·Mustafa Oguzhan Caglayan
May 22, 2007·Analytical Chemistry·R K Gilpin, C S Gilpin
Mar 16, 2007·Journal of the American Chemical Society·Noemí de-los-Santos-AlvarezPaulino Tuñón-Blanco

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