Analysis of neurogenesis during experimental autoimmune encephalomyelitis reveals pitfalls of bioluminescence imaging

PloS One
Ilya AyzenbergIngo Kleiter

Abstract

Bioluminescence imaging is a sensitive approach for longitudinal neuroimaging. Transgenic mice expressing luciferase under the promoter of doublecortin (DCX-luc), a specific marker of neuronal progenitor cells (NPC), allow monitoring of neurogenesis in living mice. Since the extent and time course of neurogenesis during autoimmune brain inflammation are controversial, we investigated neurogenesis in MOG-peptide induced experimental allergic encephalomyelitis (EAE) using DCX-luc reporter mice. We observed a marked, 2- to 4-fold increase of the bioluminescence signal intensity 10 days after EAE induction and a gradual decline 1-2 weeks thereafter. In contrast, immunostaining for DCX revealed no differences between EAE and control mice 2 and 4 weeks after immunization in zones of adult murine neurogenesis such as the dentate gyrus. Ex vivo bioluminescence imaging showed similar luciferase expression in brain homogenates of EAE and control animals. Apart from complete immunization including MOG-peptide also incomplete immunization with complete Freund´s adjuvant and pertussis toxin resulted in a rapid increase of the in vivo bioluminescence signal. Blood-brain barrier (BBB) leakage was demonstrated 10 days after both complete and i...Continue Reading

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Citations

Aug 1, 2015·ACS Chemical Neuroscience·David M Mofford, Stephen C Miller
Sep 2, 2016·Scientific Reports·Simone E HieberBert Müller
Oct 27, 2016·Journal of Neuroscience Research·Aggeliki GiannakopoulouNikolaos Grigoriadis
Feb 16, 2018·Journal of Neuroscience Research·Ivana BjelobabaIrena Lavrnja
May 19, 2019·Disease Models & Mechanisms·Liesbeth VanherpGreetje Vande Velde
Dec 5, 2019·Proceedings of the National Academy of Sciences of the United States of America·Steffen HaupeltshoferIngo Kleiter
Jul 30, 2019·Frontiers in Neuroscience·Judith MetzdorfIngo Kleiter

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Methods Mentioned

BETA
transgenic
fluorescence microscopy
bioluminescence

Software Mentioned

GraphPad Prism
DCX
ImageJ
LIVINGIMAGE
luc

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