PMID: 2495025Apr 12, 1989Paper

Analysis of nonpolar insertion mutations in the trfA gene of IncP plasmid RK2 which affect its broad-host-range property

Biochimica Et Biophysica Acta
V Shingler, C M Thomas

Abstract

Replication of broad-host-range plasmid RK2 requires the protein product(s) of the plasmid-encoded trfA gene to initiate replication at oriV, the vegetative replication origin. The trfA gene contains two translational starts which direct translation of two polypeptides, of 382 and 285 amino acids, which differ by the 97 amino acids at their N-terminus. Nonpolar insertions which abolish expression of the larger TrfA polypeptide but otherwise retain the trfA gene's normal expression signals severely reduce plasmid replication efficiency in Pseudomonas aeruginosa and to a lesser extent in Pseudomonas putida, but have very little effect in Escherichia coli. This indicates that the organization of the trfA gene, producing two polypeptides products, plays an important part in the broad-host-range of plasmid RK2 by providing a degree of flexibility in the way the plasmid's replication system interacts with host biochemistry.

References

Apr 29, 1977·Molecular & General Genetics : MGG·R MeyerD R Helinski
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D H Figurski, D R Helinski
Jan 1, 1987·Annual Review of Microbiology·C M Thomas, C A Smith
Jan 1, 1984·Molecular & General Genetics : MGG·V Shinger, C M Thomas
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E ScherzingerM Bagdasarian
May 25, 1984·Journal of Molecular Biology·C A Smith, C M Thomas

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Citations

Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·A BlasinaD R Helinski
Jan 10, 2012·Journal of Bacteriology·Hirokazu YanoEva M Top
Dec 3, 2014·Plasmid·Elzbieta ZabrockaIgor Konieczny
Jul 6, 2010·Plasmid·Katarzyna KolatkaIgor Konieczny
Jul 28, 2005·Molecular Microbiology·Yanina R SevastsyanovichChristopher M Thomas
Mar 22, 1996·The Journal of Biological Chemistry·A E ToukdarianS Perri

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