Analysis of orbital T cells in thyroid-associated ophthalmopathy

Clinical and Experimental Immunology
G FörsterG Kahaly

Abstract

Thyroid-associated ophthalmopathy (TAO) has a major effect on the two compartments of the retro-orbital (RO) space, leading to enlargement of the extraocular muscles and other RO tissues. T lymphocyte infiltration of RO tissue is a characteristic feature of TAO and there is current interest in whether these T cells are specifically and selectively reactive to RO tissue itself. We recently established 18 T cell lines (TCL) from RO adipose/connective tissue of six patients with severe TAO by using IL-2, anti-CD3 antibodies and irradiated autologous peripheral blood mononuclear cells (PBMC) to maintain the growth of T cells reactive to autologous RO tissue protein fractions. Here we report on the phenotype characteristics and cytokine gene expression profiles of these orbital TCL and on their immunoreactivity to the organ-specific thyroid antigens thyrotropin receptor (TSH-R), thyroidal peroxidase (TPO) and thyroglobulin (TG). Flow cytometry revealed that 10 TCL were predominantly of CD4+ phenotype, three being mostly CD8+ and five neither CD4+ nor CD8+. Analysis with reverse transcriptase-polymerase chain reaction (RT-PCR) of cytokine gene expression revealed both Th1- and Th2-like products in all TCL: IL-2 product (in 17 TCL), i...Continue Reading

References

Jan 1, 1992·Autoimmunity·G KahalyM Schuler
Jul 6, 1991·Lancet·A P Weetman
May 1, 1991·The Journal of Clinical Endocrinology and Metabolism·T J SmithM Cheavens
Jan 1, 1994·Thyroid : Official Journal of the American Thyroid Association·C StoverG Kahaly
Oct 1, 1994·Clinical Immunology and Immunopathology·G KahalyH P Dienes
Nov 1, 1993·The Journal of Clinical Endocrinology and Metabolism·M de CarliG del Prete
Dec 1, 1993·Endocrine Reviews·H B Burch, L Wartofsky
May 1, 1994·Clinical and Experimental Immunology·K ArnoldA P Weetman
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·E M SchwarzB R Bloom
Jan 1, 1993·Annual Review of Immunology·K W MooreT R Mosmann
Nov 11, 1993·The New England Journal of Medicine·R S Bahn, A E Heufelder
Jan 1, 1993·European Journal of Clinical Investigation·A E Heufelder, R S Bahn
Mar 22, 1996·Science·T ForsthuberP V Lehmann
Aug 1, 1996·The Journal of Infectious Diseases·D B CorryJ N Galgiani
Dec 1, 1995·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·E OttoG Kahaly
Aug 1, 1996·The Journal of Clinical Endocrinology and Metabolism·E A OttoG J Kahaly
Aug 1, 1997·Clinical and Experimental Immunology·A PappaS Lightman

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Citations

May 17, 2001·Therapeutic Apheresis : Official Journal of the International Society for Apheresis and the Japanese Society for Apheresis·H ProphetW Ramlow
Apr 6, 2001·Thyroid : Official Journal of the American Thyroid Association·M MarinòL Chiovato
Apr 16, 2002·Thyroid : Official Journal of the American Thyroid Association·Tomasz BednarczukJanusz Nauman
Apr 16, 2002·Thyroid : Official Journal of the American Thyroid Association·Yuji HiromatsuEri Soejima
Jan 13, 2001·Cancer Biotherapy & Radiopharmaceuticals·G J FörsterG J Kahaly
Aug 21, 2008·Thyroid : Official Journal of the American Thyroid Association·Vibhavari NaikRaymond S Douglas
May 8, 2009·Folia Histochemica Et Cytobiologica·Przemysław PawlowskiMaria Gorska
Nov 15, 2015·The British Journal of Ophthalmology·Jwu Jin KhongDinesh Selva
Apr 14, 2010·Survey of Ophthalmology·Vibhavari M NaikRaymond S Douglas
Oct 5, 2001·Expert Opinion on Pharmacotherapy·J J Díez
May 2, 2018·JAMA Ophthalmology·Giovanna Rotondo DottoreMichele Marinò
May 18, 2018·Ophthalmic Plastic and Reconstructive Surgery·Tanja Diana, George J Kahaly
Apr 22, 1999·Thyroid : Official Journal of the American Thyroid Association·D YangK Nonaka
Feb 24, 2001·Critical Reviews in Clinical Laboratory Sciences·M YamadaJ R Wall
Dec 6, 2018·The Journal of Clinical Endocrinology and Metabolism·Sijie FangXianqun Fan
Dec 13, 2003·Endocrine Reviews·Bellur S PrabhakarTerry J Smith
Mar 17, 2000·The Journal of Clinical Endocrinology and Metabolism·Y HiromatsuY Inoue
Sep 21, 2019·Journal of Endocrinological Investigation·Y P HaiG J Kahaly
Mar 9, 2018·International Ophthalmology Clinics·Yao WangPradeep Mettu
May 4, 2007·Endocrine Reviews·Mario RotondiPaola Romagnani
Apr 27, 2000·Endocrine Reviews·L BartalenaC Marcocci
Sep 13, 2003·The Journal of Clinical Endocrinology and Metabolism·Seema Kumar, Rebecca S Bahn
Aug 5, 2020·Journal of Endocrinological Investigation·T DianaG J Kahaly
May 29, 2004·Journal of Endocrinological Investigation·R A Ajjan, A P Weetman
Mar 30, 2021·Frontiers in Endocrinology·Lara Frommer, George J Kahaly
Apr 20, 2021·Frontiers in Endocrinology·Sijie FangXianqun Fan

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