Analysis of oxysterol binding protein homologue Kes1p function in regulation of Sec14p-dependent protein transport from the yeast Golgi complex

The Journal of Cell Biology
Xinmin LiVytas A Bankaitis

Abstract

Oxysterol binding proteins (OSBPs) comprise a large conserved family of proteins in eukaryotes. Their ubiquity notwithstanding, the functional activities of these proteins remain unknown. Kes1p, one of seven members of the yeast OSBP family, negatively regulates Golgi complex secretory functions that are dependent on the action of the major yeast phosphatidylinositol/phosphatidylcholine Sec14p. We now demonstrate that Kes1p is a peripheral membrane protein of the yeast Golgi complex, that localization to the Golgi complex is required for Kes1p function in vivo, and that targeting of Kes1p to the Golgi complex requires binding to a phosphoinositide pool generated via the action of the Pik1p, but not the Stt4p, PtdIns 4-kinase. Localization of Kes1p to yeast Golgi region also requires function of a conserved motif found in all members of the OSBP family. Finally, we present evidence to suggest that Kes1p may regulate adenosine diphosphate-ribosylation factor (ARF) function in yeast, and that it may be through altered regulation of ARF that Kes1p interfaces with Sec14p in controlling Golgi region secretory function.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Dec 1, 1989·The Journal of Cell Biology·A E ClevesV A Bankaitis
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
May 17, 1994·Biochemistry·G Dormán, G D Prestwich
Feb 1, 1997·Photochemistry and Photobiology·G D PrestwichA Chaudhary
May 9, 1998·The Journal of Biological Chemistry·A ChaudharyG D Prestwich
May 14, 1998·Chemistry & Biology·A ChaudharyG D Prestwich
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z XieV A Bankaitis
Dec 16, 1998·Science·UNKNOWN C. elegans Sequencing Consortium
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·M S Brown, J L Goldstein
Dec 10, 1999·Nature Cell Biology·C Walch-Solimena, P Novick
Jul 1, 1991·Trends in Cell Biology·A ClevesV Bankaitis

❮ Previous
Next ❯

Citations

Mar 16, 2013·Trends in Cell Biology·Yeun Ju KimTamas Balla
Oct 18, 2002·Current Opinion in Cell Biology·Maria De MatteisDaniela Corda
Aug 2, 2003·Current Opinion in Cell Biology·Vytas A Bankaitis, Andrew J Morris
Apr 27, 2004·Nature Cell Biology·Anna GodiM Antonietta De Matteis
Nov 10, 2009·Nature Cell Biology·Paramasivam NatarajanTodd R Graham
Sep 9, 2010·Nature Reviews. Molecular Cell Biology·Sima Lev
Jan 16, 2007·Nature Structural & Molecular Biology·Guillaume DrinBruno Antonny
Aug 24, 2010·The Journal of Biological Chemistry·Marissa A LeBlanc, Christopher R McMaster
Oct 11, 2008·The Journal of Biological Chemistry·Valérie Prouzet-MauléonFrançois Doignon
Feb 16, 2012·The Journal of Biological Chemistry·Christopher T BehAnant K Menon
Oct 7, 2003·Molecular Biology of the Cell·Simon A RudgeJoAnne Engebrecht
Feb 22, 2008·Molecular Biology of the Cell·Lars DemmelChristiane Walch-Solimena
May 9, 2006·Current Opinion in Lipidology·John B Massey
Apr 2, 2011·Cold Spring Harbor Perspectives in Biology·Peter Mayinger
Nov 2, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Gabriel SchaafVytas A Bankaitis
Nov 22, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Jihui RenManish C Pathak
Mar 31, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Vesa M Olkkonen, Timothy P Levine
Mar 31, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Sheri M Routt, Vytas A Bankaitis
Oct 20, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Gregory D Fairn, Christopher R McMaster
Jul 24, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Marissa A LeBlanc, Christopher R McMaster
Aug 14, 2009·Physiology·Tamas BallaYeun Ju Kim
Mar 20, 2008·Molecular Neurodegeneration·Celina V ZerbinattiBenjamin Wolozin
May 24, 2011·Clinical Lipidology·Aaron H NileAby Grabon
Mar 29, 2011·Annual Review of Biochemistry·Bruno Antonny
Dec 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·Tomasz J ProszynskiChristiane Walch-Solimena
Mar 13, 2014·Annual Review of Biochemistry·Guillaume Drin
Sep 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Gregory D FairnChristopher R McMaster
Jul 7, 2009·Annual Review of Cell and Developmental Biology·Sumana Raychaudhuri, William A Prinz
Oct 20, 2006·Nature Chemical Biology·Kristina E IleVytas A Bankaitis
Dec 3, 2013·Nature Chemical Biology·Aaron H NileVytas A Bankaitis
May 3, 2003·Genome Research·Austin L Hughes, Robert Friedman
Jan 4, 2013·Cellular and Molecular Life Sciences : CMLS·Bruno MesminGuillaume Drin
Dec 14, 2005·FEBS Letters·J Pedro Fernández-Murray, Christopher R McMaster
Dec 17, 2009·The Journal of Cell Biology·Timothy A SchulzWilliam A Prinz

❮ Previous
Next ❯

Methods Mentioned

BETA
GTPase
fluorescence microscopy
nucleotide exchange
column chromatography

Software Mentioned

BLAST
IP Lab
Adobe Photoshop

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.