Mar 25, 2020

Analysis of p67 allelic sequences reveals a subtype of allele type 1 unique to buffalo-derived Theileria parva parasites from southern Africa

BioRxiv : the Preprint Server for Biology
Lubembe D. MukolweK. P. Sibeko-Matjila


East Coast fever (ECF) and Corridor disease (CD) caused by cattle- and buffalo-derived T. parva respectively are the most economically important tick-borne diseases of cattle in the affected African countries. The p67 gene has been evaluated as a recombinant subunit vaccine against East Coast fever (ECF), and for discrimination of T. parva parasites causing ECF and Corridor disease (CD). The p67 allele type 1 was first identified in cattle-derived T. parva parasites from east Africa, where parasites possessing this allele type have been associated with ECF. Subsequent characterization of buffalo-derived T. parva parasites from South Africa where ECF was eradicated, revealed the presence of a similar allele type, raising concerns as to whether or not allele type 1 from parasites from the two regions is identical. A 900 bp central fragment of the gene encoding p67 was PCR amplified from T. parva DNA extracted from blood collected from cattle and buffalo in South Africa, Mozambique, Kenya, Tanzania and Uganda, followed by DNA sequence analysis. Four p67 allele types previously described were identified. A subtype of p67 allele type 1 was identified in parasites from clinical cases of CD and buffalo from southern Africa. Notably, p...Continue Reading

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Mentioned in this Paper

Gene Annotation
Genome Sequencing
Genome, Human
Sequence Alignment
Molecular Assembly/Self Assembly

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