Analysis of paralytic shellfish poisoning toxin congeners by a sodium channel receptor binding assay

Toxicon : Official Journal of the International Society on Toxinology
Gires UsupBoon-Koon Ng

Abstract

This study was carried out to characterize the detection and quantitation of several paralytic shellfish poisoning (PSP) toxin congeners using a receptor binding assay (RBA). This involved competitive binding of the toxin congeners against tritium-labeled STX for receptor sites on rat brain sodium channels. Competitive binding curves were described by a four-parameter logistic equation. Half-saturation values (EC(50)) ranged from 4.38 nM for STX to 142 nM for GTX5. Receptor binding affinity was in the order STX>GTX1/4>neoSTX>GTX2/3>dcSTX>GTX5, and this was similar to the order of mouse toxicity of these congeners. Predicted toxin concentrations from observed STXeq values and EC(50) ratios relative to STX were within 20% or better of the actual concentrations used in the assay. In contrast predicted toxin concentrations using mouse toxicity ratios relative to STX did not provide a good match to actual concentrations, except for GTX1/4. This study has shown that the rat brain sodium channel RBA will provide a reliable integration of total toxicity of various PSP toxin congeners present in a sample.

References

Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·E MoczydlowskiG R Strichartz
Nov 1, 1981·Journal of Agricultural and Food Chemistry·A A Genenah, Y Shimizu
May 1, 1997·Toxicon : Official Journal of the International Society on Toxinology·G J DoucetteF M Van Dolah

❮ Previous
Next ❯

Citations

Mar 25, 2011·Journal of Toxicology and Environmental Health. Part a·David R RuebhartGeoffrey K Eaglesham
Mar 31, 2006·Natural Product Reports·Lyndon E Llewellyn
Jan 27, 2010·Analytical and Bioanalytical Chemistry·A R HumpageS M Froscio
Dec 29, 2009·Toxicon : Official Journal of the International Society on Toxinology·Stacey M Etheridge
May 20, 2009·Toxicon : Official Journal of the International Society on Toxinology·Pedro Reis CostaKathi A Lefebvre
Aug 27, 2009·Toxicon : Official Journal of the International Society on Toxinology·Cedric RobillotLyndon E Llewellyn
Aug 7, 2007·Toxicon : Official Journal of the International Society on Toxinology·Lyndon E Llewellyn
Jun 24, 2009·Journal of Separation Science·Weimin WuJohn P Giesy
Oct 11, 2007·Angewandte Chemie·Osamu IwamotoKazuo Nagasawa
Dec 2, 2008·Chemistry, an Asian Journal·Osamu IwamotoKazuo Nagasawa
Mar 13, 2012·Toxicon : Official Journal of the International Society on Toxinology·Xiao-Wei Zhang, Zhao-Xiang Zhang
Sep 26, 2013·Toxicon : Official Journal of the International Society on Toxinology·Rex MundayMichael A Quilliam
Dec 30, 2014·Ecotoxicology and Environmental Safety·De Lu ZhangYong Ding Liu
Jul 5, 2016·Toxicon : Official Journal of the International Society on Toxinology·Keith HarrisonAndrew D Turner
Oct 14, 2017·Marine Drugs·Lorena M Durán-Riveroll, Allan D Cembella
Jan 4, 2019·Chembiochem : a European Journal of Chemical Biology·April L Lukowski, Alison R H Narayan
Oct 23, 2019·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Ambbar Aballay-GonzálezAllisson Astuya
Aug 19, 2017·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Shiyamalie R RuberuRaphael M Kudela
May 16, 2006·Chemical Research in Toxicology·Lyndon E Llewellyn
Sep 8, 2018·Journal of the American Chemical Society·April L LukowskiAlison R H Narayan
Apr 16, 2019·ACS Chemical Biology·April L LukowskiAlison R H Narayan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.