Analysis of partitioning of organic compounds and proteins in aqueous polyethylene glycol-sodium sulfate aqueous two-phase systems in terms of solute-solvent interactions

Journal of Chromatography. a
Nuno R da SilvaB Y Zaslavsky

Abstract

Partition behavior of nine small organic compounds and six proteins was examined in poly(ethylene glycol)-8000-sodium sulfate aqueous two-phase systems containing 0.5M osmolyte (sorbitol, sucrose, trehalose, TMAO) and poly(ethylene glycol)-10000-sodium sulfate system, all in 0.01M sodium phosphate buffer, pH 6.8. The differences between the solvent properties of the coexisting phases (solvent dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) were characterized with solvatochromic dyes using the solvatochromic comparison method. Differences between the electrostatic properties of the phases were determined by analysis of partitioning of sodium salts of dinitrophenylated (DNP-) amino acids with aliphatic alkyl side-chain. It was found out that the partition coefficient of all compounds examined (including proteins) may be described in terms of solute-solvent interactions. The results obtained in the study show that solute-solvent interactions of nonionic organic compounds and proteins in polyethylene glycol-sodium sulfate aqueous two-phase system differ from those in polyethylene glycol-dextran system.

References

Aug 15, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·J G HuddlestonR D Rogers
Jun 25, 2004·Journal of Chromatography. a·Michael H AbrahamAndreas M Zissimos
Dec 22, 2009·The Journal of Physical Chemistry. B·Pedro P MadeiraBoris Y Zaslavsky
Aug 19, 2010·Biotechnology and Bioengineering·Stefan A OelmeierJürgen Hubbuch
Feb 4, 2011·Journal of Chromatography. a·Pedro P MadeiraBoris Y Zaslavsky
Jun 15, 2011·Journal of Chromatography. a·Luisa A FerreiraBoris Y Zaslavsky
Aug 23, 2011·Physical Chemistry Chemical Physics : PCCP·M A Ab RaniR Wilding
Dec 27, 2011·Journal of Chromatography. a·Luisa A FerreiraBoris Y Zaslavsky
Feb 14, 2012·Journal of Chromatography. a·Pedro P MadeiraBoris Y Zaslavsky
Apr 13, 2012·Journal of Chromatography. a·Juan A Asenjo, Barbara A Andrews
Dec 12, 2012·Journal of Chromatography. a·Pedro P MadeiraBoris Y Zaslavsky
Jun 21, 2013·Journal of Biomolecular Structure & Dynamics·Pedro P MadeiraBoris Y Zaslavsky
Aug 9, 2013·Biotechnology Progress·João Vitor Dutra MolinoMaria Silvia Viccari Gatti
Nov 26, 2013·Journal of Chromatography. a·Pedro P MadeiraBoris Y Zaslavsky
Jun 7, 2014·Journal of Pharmaceutical Sciences·Michael H Abraham
Sep 27, 2014·Physical Chemistry Chemical Physics : PCCP·L A FerreiraB Y Zaslavsky
Dec 3, 2014·Protein Expression and Purification·Rocío L PérezGisela Tubio
Jan 3, 2015·Journal of Bioscience and Bioengineering·Yin Hui ChowTau Chuan Ling
Feb 25, 2015·Journal of Chromatography. a·Luisa A FerreiraBoris Y Zaslavsky
Mar 5, 2015·Biotechnology Progress·Mario A Torres-AcostaNigel J Titchener-Hooker
Mar 24, 2015·Chemistry Central Journal·Jean-Claude BradleyAndrew Sid Lang
Apr 11, 2015·Biotechnology Progress·Jan MuendgesTim Zeiner
Apr 30, 2015·Biotechnology Advances·J F BuyelR Fischer
May 12, 2015·Biotechnology Progress·Patrick DiederichJürgen Hubbuch
Jun 25, 2015·Journal of Chromatography. a·Johannes GollMirjana Minceva
Jul 15, 2015·Journal of Chromatography. a·Pedro P MadeiraBoris Y Zaslavsky

❮ Previous
Next ❯

Citations

Dec 28, 2017·Advanced Healthcare Materials·Alyne G TeixeiraJohn P Frampton
Sep 13, 2019·Biomolecules·Boris Y ZaslavskyVladimir N Uversky
Feb 13, 2020·Physical Chemistry Chemical Physics : PCCP·Amber R TitusBoris Y Zaslavsky
Aug 23, 2019·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Pratik U JoshiCaryn L Heldt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.