Jul 1, 1976

Analysis of passive and active electrophysiologic properties of neurons in mammalian nodose ganglia maintained in vitro

Journal of Neurophysiology
R A Jaffe, S R Sampson

Abstract

1. We studied the passive and active electrical properties of the soma membrane of neurons in nodose ganglia removed from cats and rabbits and maintained in vitro. The ganglia were superfused at 37 degrees C with a solution formulated to approximate the extracellular fluid of each species. The solution was buffered to pH 7.34, continuously equilibrated with 95% O2 and 5% CO2, and contained dialyzed calf serum and glucose. We also examined these properties in nodose ganglion neurons in vivo. Intracellular recordings were obtained with glass micropipettes filled with either 3 M KCl or 5 M K acetate. 2. We determined mean values for a variety of passive and active electrophysiologic properties. Values obtained in vitro did not differ significantly from those obtained in vivo. Based on the passive electrical properties of the soma membrane, neurons in the nodose ganglion appear to be a uniform population, despite the different sensory modalities conveyed by the afferent fibers. 3. Cell bodies of neurons generated action potentials in response to impulses in their afferent fibers. Somatic spikes could be evoked by stimulation of either the supranodose or infranodose vagus nerve, and an inflection point could be seen on their rising ...Continue Reading

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Mentioned in this Paper

Buffers
Structure of Calf of Leg
Protoplasm
Resting Potentials
Neurons
Cattle calf (organism)
Action Potentials
Bos taurus
Extracellular Fluid
Vagus Nerve Structure

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