Analysis of phospholipid transfer during HDL binding to platelets using a fluorescent analog of phosphatidylcholine

Thrombosis Research
F Martin-NizardC Delbart

Abstract

Electron microscopic examination of the interaction of gold labelled HDL with platelets indicates that the internalized lipoprotein becomes closely associated with surface connected canaliculae and endocytic vacuoles. At the same time granule centralization occurs. Using fluorescent derivatives of naturally occurring lipids we have further investigated lipid exchange between HDL and platelets. Analogs of phosphatidylcholine containing fluorescent fatty acids are rapidly transferred from the lipoproteins to the cells and remain at the plasma membrane as long as they are kept at 4 degrees C. However when the platelets are warmed to 37 degrees C, a rapid degradation of the fluorescent lipids occurs, generating fluorescent diacylglycerol as a consequence of the activation of platelet enzymes.

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Citations

Oct 17, 1989·Biochimica Et Biophysica Acta·F Martin-NizardC Delbart
Oct 3, 2003·Biochemical and Biophysical Research Communications·Evelize FollyGeorgia C Atella
Aug 1, 2006·Pathophysiology of Haemostasis and Thrombosis·Elisabeth KollerFranz Koller
Nov 18, 2008·Acta Tropica·Georgia C AtellaMário A C Silva-Neto
Sep 15, 1990·Thrombosis Research·H NazihC Delbart
Dec 31, 1990·Biochemical and Biophysical Research Communications·R M CataliotoR Negrel
Mar 3, 2012·Experimental Parasitology·Nuccia N T De CiccoGeorgia C Atella
Nov 27, 2007·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·A A XimenesG C Atella
Jul 1, 1992·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·I VirgoliniH Sinzinger
Jan 1, 1987·Annals of the New York Academy of Sciences·P C AdamsV Fuster
May 3, 2006·Insect Biochemistry and Molecular Biology·Georgia C AtellaMohammed Shahabuddin

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