Analysis of Phosphorylation of the Receptor-Like Protein Kinase HAESA during Arabidopsis Floral Abscission

PloS One
Isaiah TaylorJohn C Walker

Abstract

Receptor-like protein kinases (RLKs) are the largest family of plant transmembrane signaling proteins. Here we present functional analysis of HAESA, an RLK that regulates floral organ abscission in Arabidopsis. Through in vitro and in vivo analysis of HAE phosphorylation, we provide evidence that a conserved phosphorylation site on a region of the HAE protein kinase domain known as the activation segment positively regulates HAE activity. Additional analysis has identified another putative activation segment phosphorylation site common to multiple RLKs that potentially modulates HAE activity. Comparative analysis suggests that phosphorylation of this second activation segment residue is an RLK specific adaptation that may regulate protein kinase activity and substrate specificity. A growing number of RLKs have been shown to exhibit biologically relevant dual specificity toward serine/threonine and tyrosine residues, but the mechanisms underlying dual specificity of RLKs are not well understood. We show that a phospho-mimetic mutant of both HAE activation segment residues exhibits enhanced tyrosine auto-phosphorylation in vitro, indicating phosphorylation of this residue may contribute to dual specificity of HAE. These results a...Continue Reading

References

Oct 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S S TaylorT Hunter
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
May 25, 1999·BioTechniques·L Gama, G E Breitwieser
Aug 30, 2001·Proceedings of the National Academy of Sciences of the United States of America·S H Shiu, A B Bleecker
May 23, 2002·Cell·Morgan Huse, John Kuriyan
Aug 2, 2002·Cell·Kyoung Hee Nam, Jianming Li
Jun 3, 2004·Electrophoresis·Giovanni CandianoPier Giorgio Righetti
Dec 2, 2004·The Journal of Biological Chemistry·Satoko Yoshida, Martin Parniske
Feb 18, 2006·Plant Methods·Kevin A LeaseJohn C Walker
Jul 13, 2007·Nature·Delphine ChinchillaThomas Boller
Jul 13, 2007·Proceedings of the National Academy of Sciences of the United States of America·Antje HeeseJohn P Rathjen
Jul 14, 2007·Trends in Biochemical Sciences·Antony W OliverLaurence H Pearl
Jan 24, 2008·Plant Methods·Manu J DubinGiovanna Benvenuto
Sep 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sung Ki ChoJohn C Walker
Jan 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Man-Ho OhSteven C Huber
Mar 17, 2010·The Plant Journal : for Cell and Molecular Biology·Michael W LewisSarah J Liljegren
Jun 10, 2010·Bioscience, Biotechnology, and Biochemistry·Shinya NakamuraSumie Ishiguro
Oct 26, 2010·Trends in Biochemical Sciences·Susan S Taylor, Alexandr P Kornev
Jan 6, 2011·The Journal of Biological Chemistry·Dörte Klaus-HeisenJulie V Cullimore
Jun 2, 2011·Plant Physiology·Christian A BurrSarah J Liljegren
Dec 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Man-Ho OhSteven C Huber

❮ Previous
Next ❯

Citations

Jan 21, 2006·PLoS Pathogens·Christopher Dardick, Pamela Ronald
Dec 19, 2009·European Journal of Cell Biology·Sandra PostelThorsten Nürnberger
Oct 12, 2010·Current Opinion in Plant Biology·Jia Li
Jul 24, 2007·Trends in Cell Biology·Richard B MeagherElizabeth C McKinney
Nov 8, 2016·Frontiers in Plant Science·William D GronerSarah J Liljegren
Feb 17, 2017·Journal of Experimental Botany·Pawel Z KosentkaElena D Shpak
Jun 7, 2020·International Journal of Molecular Sciences·Jismon JoseSwarup Roy Choudhury
Jun 24, 2020·International Journal of Molecular Sciences·Juan Mao, Jianming Li
Dec 29, 2020·Frontiers in Plant Science·Yali SunJin-Gui Chen
Jul 24, 2021·The Biochemical Journal·Henning MühlenbeckCyril Zipfel
Sep 18, 2021·Proceedings of the National Academy of Sciences of the United States of America·Kyle W BenderCyril Zipfel
Dec 10, 2021·Omics : a Journal of Integrative Biology·Pratigya Subba, Thottethodi Subrahmanya Keshava Prasad

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR
affinity purification

Software Mentioned

ImageQ
Google Scholar
ClustalW
Adobe Photoshop

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.