Analysis of photobleaching in single-molecule multicolor excitation and Förster resonance energy transfer measurements

The Journal of Physical Chemistry. a
C EggelingClaus A M Seidel

Abstract

Dye photobleaching is a major constraint of fluorescence readout within a range of applications. In this study, we investigated the influence of photobleaching in fluorescence experiments applying multicolor laser as well as Förster resonance energy transfer (FRET) mediated excitation using several red-emitting dyes frequently used in multicolor experiments or as FRET acceptors. The chosen dyes (cyanine 5 (Cy5), MR121, Alexa660, Alexa680, Atto647N, Atto655) have chemically distinct chromophore systems and can be excited at 650 nm. Several fluorescence analysis techniques have been applied to detect photobleaching and to disclose the underlying photophysics, all of which are based on single-molecule detection: (1) fluorescence correlation spectroscopy (FCS) of bulk solutions, (2) fluorescence cross-correlation of single-molecule trajectories, and (3) multiparameter fluorescence detection (MFD) of single-molecule events. The maximum achievable fluorescence signals as well as the survival times of the red dyes were markedly reduced under additional laser irradiation in the range of 500 nm. Particularly at excitation levels at or close to saturation, the 500 nm irradiation effectively induced transitions to higher excited electroni...Continue Reading

References

Jan 1, 1992·Methods in Enzymology·R M Clegg
May 1, 1990·Journal of Photochemistry and Photobiology. B, Biology·E V Khoroshilova, D N Nikogosyan
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·M Eigen, R Rigler
Feb 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·S DamjanovichT M Jovin
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·C EggelingC A Seidel
May 22, 1989·Physical Review Letters·W E Moerner, L Kador
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·A A DenizP G Schultz
Jul 2, 1999·Biophysical Journal·Y ChenE Gratton
Oct 26, 1999·Current Biology : CB·F S Wouters, P I Bastiaens
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·P KaskK Gall
Apr 28, 2001·Annual Review of Physical Chemistry·A A DenizS Weiss
Sep 18, 2001·Methods : a Companion to Methods in Enzymology·T Ha
Dec 26, 2001·Chemical Reviews·W P AmbroseR A Keller
Sep 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tobias KohlPetra Schwille
Feb 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·P J RothwellC A M Seidel
Mar 5, 2003·The Journal of Cell Biology·Rajesh Babu Sekar, Ammasi Periasamy
Jul 15, 2003·Physical Review Letters·Taekjip Ha, Jian Xu
Oct 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Johan HofkensFrans De Schryver
Nov 5, 2003·Nature Biotechnology·Elizabeth A Jares-Erijman, Thomas M Jovin
Dec 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·M MargittaiC A M Seidel
Jan 20, 2004·Nature Structural & Molecular Biology·Manuel DiezPeter Gräber
Mar 12, 2004·Annual Review of Physical Chemistry·X S Xie, J K Trautman
Jun 4, 2004·Proceedings of the National Academy of Sciences of the United States of America·Achillefs N KapanidisShimon Weiss
Jun 11, 2004·Biophysical Journal·Tomasz Zal, Nicholas R J Gascoigne
Dec 15, 2004·Biophysical Journal·Yan ChenJoachim D Müller
Mar 18, 2005·Journal of the American Chemical Society·Mike HeilemannPhilip Tinnefeld
Mar 24, 2005·Physical Review Letters·Mark BatesXiaowei Zhuang
May 11, 2005·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Christian EggelingClaus A M Seidel
Aug 23, 2005·Biophysical Journal·Barbara K MüllerDon C Lamb

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Citations

Jun 16, 2011·European Biophysics Journal : EBJ·Arne WernerUlrich Hahn
Jul 5, 2007·Journal of Fluorescence·W J A KoopmansJ van Noort
Mar 23, 2007·Journal of the American Chemical Society·Xiangxu KongShimon Weiss
Sep 24, 2009·Journal of the American Chemical Society·Edward A LemkeAshok A Deniz
Aug 4, 2006·The Journal of Physical Chemistry. a·David S Talaga
Nov 4, 2011·Organic Letters·Steven M Pauff, Stephen C Miller
Jul 6, 2010·Molecular BioSystems·Yann Gambin, Ashok A Deniz
May 24, 2007·Journal of the Royal Society, Interface·Ashok A DenizEdward A Lemke
Sep 13, 2006·Journal of Biomedical Optics·Marcel LeuteneggerTheo Lasser
Apr 17, 2008·Annual Review of Biochemistry·Chirlmin JooTaekjip Ha
May 5, 2012·PloS One·Christopher J TynanStephen E D Webb
Mar 13, 2012·Annual Review of Physical Chemistry·Taekjip Ha, Philip Tinnefeld
Jul 27, 2010·Biophysical Journal·Agnes SzabóPeter Nagy
Oct 22, 2009·Biophysical Journal·Sergi Padilla-ParraMarc Tramier
May 21, 2011·Journal of Molecular Biology·Dana KahraChristian Gerhard Hübner
May 23, 2015·Quarterly Reviews of Biophysics·Christian EggelingStefan W Hell
Jan 1, 2014·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Jean-Maxime GuayNicolas Abatzoglou
Aug 15, 2008·Neuron·Antoine Triller, Daniel Choquet
Mar 7, 2017·Chemical Reviews·Hans Blom, Jerker Widengren
Nov 20, 2015·Physical Chemistry Chemical Physics : PCCP·Daniel NettelsBenjamin Schuler
Jul 1, 2017·The Journal of Organic Chemistry·H M Dhammika BandaraWilliam R Kobertz
Aug 24, 2017·Biomaterials Science·Anthony N Cauley, James N Wilson
Jul 8, 2008·Angewandte Chemie·Jan VogelsangPhilip Tinnefeld
Jul 12, 2008·Angewandte Chemie·Frank HillgerBenjamin Schuler
Sep 1, 2012·Chembiochem : a European Journal of Chemical Biology·Søren Preus, L Marcus Wilhelmsson
Mar 8, 2008·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Christian RingemannChristian Eggeling
Apr 15, 2014·Microscopy Research and Technique·Andreas SchönleChristian Eggeling
Feb 3, 2012·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Elana M S StennettMarcia Levitus
Jul 4, 2008·Annals of the New York Academy of Sciences·Suzana M Andrade, Sílvia M B Costa
Apr 2, 2009·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Stefanie Weidtkamp-PetersClaus A M Seidel
Dec 2, 2009·Chemistry : a European Journal·Kirill KolmakovStefan W Hell
Jul 16, 2010·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Jan VogelsangPhilip Tinnefeld
Feb 10, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Michael Börsch, Jörg Wrachtrup
Dec 24, 2011·Physical Chemistry Chemical Physics : PCCP·A L JouonangY Mély
Nov 2, 2013·Chemical Society Reviews·Qinsi ZhengScott C Blanchard
Nov 6, 2013·Chemical Society Reviews·Bolesław Kozankiewicz, Michel Orrit
Jan 1, 2014·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Kathrin KlehsMike Heilemann
May 31, 2019·PLoS Computational Biology·Shilo OhayonAmit Meller
Apr 10, 2014·Angewandte Chemie·Phil HolzmeisterPhilip Tinnefeld
Mar 4, 2014·Methods and Applications in Fluorescence·Lydia KisleyChristy F Landes
Mar 3, 2012·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Volodymyr KudryavtsevDon C Lamb

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