Analysis of Phylogenetic Variation of Stenotrophomonas maltophilia Reveals Human-Specific Branches

Frontiers in Microbiology
Joerg SteinmannThomas A Kohl

Abstract

Stenotrophomonas maltophilia is a non-fermenting Gram-negative bacterium that is ubiquitous in the environment. In humans, this opportunistic multi-drug-resistant pathogen is responsible for a plethora of healthcare-associated infections. Here, we utilized a whole genome sequencing (WGS)-based phylogenomic core single nucleotide polymorphism (SNP) approach to characterize S. maltophilia subgroups, their potential association with human infection, and to detect any possible transmission events. In total, 89 isolates (67 clinical and 22 environmental) from Germany were sequenced. Fully finished genomes of five strains were included in the dataset for the core SNP phylogenomic analysis. WGS data were compared with conventional genotyping results as well as with underlying disease, biofilm formation, protease activity, lipopolysaccharide (LPS) SDS-PAGE profiles, and serological specificity of an antibody raised against the surface-exposed O-antigen of strain S. maltophilia K279a. The WGS-based phylogenies grouped the strains into 12 clades, out of which 6 contained exclusively human and 3 exclusively environmental isolates. Biofilm formation and proteolytic activity did correlate neither with the phylogenetic tree, nor with the ori...Continue Reading

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Citations

Jan 16, 2019·Journal of Bacteriology·Tara GallagherKatrine Whiteson
Mar 14, 2019·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Jun LiGuangxiang Cao
Oct 30, 2019·Expert Review of Anti-infective Therapy·Samantha Flores-TreviñoElvira Garza-González
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May 28, 2021·Clinical Microbiology Reviews·Joanna S Brooke
Jan 11, 2022·Applied Biochemistry and Biotechnology·Moitreyee ChattopadhyayGautam Kumar Bagchi

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Datasets Mentioned

BETA
DSM14405

Methods Mentioned

BETA
genotyping
Protease Assay
PCR
electrophoresis

Software Mentioned

Splitstree4
FigTree
FastTree
Perl scripts
Mauve
BWA
GATK
Samtools
WGS

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