PMID: 16523689Mar 10, 2006Paper

Analysis of point mutations in BRCA1 gene using hybridization on hydrogel microchips

Molekuliarnaia biologiia
O E FedorovaT V Nasedkina

Abstract

Germ-line mutations in BRCA1 gene account for a substantial proportion of inherited breast and ovarian cancers. Identification of these mutations allows molecular diagnosis for breast cancer susceptibility. We have developed method for identification of 185delAG, 300T>G, 4153delA, 4158A>G and 5382insC mutations in BRCA1 gene using hybridization with microarray of geL-immobilized oligonucleotides (microchip). The microchip was tested with 36 control samples, carrying the above-mentioned mutations and 65 clinical cases with breast cancer. Our data demonstrated that developed microchip can be very effective and realible tool, easily introduced in ordinary medical and genetic laboratories.

References

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Citations

Jan 22, 2010·Molekuliarnaia biologiia·D V MitrofanovV V Liakhovich
Feb 5, 2008·Molekuliarnaia biologiia·A M KolchinskiĭĭA S Zasedatelev
Feb 18, 2018·The Journal of Biological Chemistry·Denis SusorovElena Alkalaeva
Feb 1, 2011·Journal of Mathematical Biology·Zhan ChenG W Wei
Oct 26, 2011·Expert Review of Molecular Diagnostics·Dmitry GryadunovAlexander Zasedatelev

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