Analysis of poly(ADP-ribose) polymerase-1 (PARP1) gene alteration in human germ cell tumor cell lines

Cancer Genetics and Cytogenetics
Hideki OginoM Masutani

Abstract

The poly(ADP-ribose) polymerase-1 protein (PARP-1) functions in DNA repair, maintenance of genomic stability, induction of cell death, and transcriptional regulation. We previously analyzed alterations of the PARP1 gene in 16 specimens of human germ cell tumors, and found a heterozygous sequence alteration that causes the amino acid substitution Met129Thr (M129T) in both tumor and normal tissues in a single patient. In this study, aberration of the PARP1 gene and protein was further analyzed in human germ cell tumor cell lines. We found a nonheterozygous sequence alteration that causes the amino acid substitution Glu251Lys (E251K) located at a conserved peptide stretch of PARP-1 in cell line NEC8. Sequencing of 95 samples from Japanese healthy volunteers revealed that all the samples were homozygous for the wild-type alleles at M129T and E251K. The M129T allele is thus suggested to be a rare single-nucleotide polymorphism (SNP). We observed a decrease in auto-poly(ADP-ribosyl)ation activity of PARP-1 proteins harboring M129T or E251K amino acid substitution, but the difference was not statistically significant. The levels of PARP-1 and poly(ADP-ribosyl)ation were heterogeneous among germ cell tumor cell lines. The SNPs of the P...Continue Reading

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Citations

Mar 15, 2013·Journal of Clinical Pathology·Michal MegoJozef Mardiak
Jun 28, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Mohammed AlanaziNarasimha Reddy Parine
Mar 20, 2018·Development Genes and Evolution·Zhen ZhangYan Huang
Jul 28, 2020·International Journal of Molecular Sciences·Liang YuLin Gao
May 30, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Atika Hazzaa AlshammariHesham Mahmoud Saeed

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